PMID- 21458234
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20110725
IS  - 1399-0020 (Electronic)
IS  - 0901-5027 (Linking)
VI  - 40
IP  - 7
DP  - 2011 Jul
TI  - In vitro investigation of the secondary palate development in two strains of 
      mice.
PG  - 737-42
LID - 10.1016/j.ijom.2011.02.020 [doi]
AB  - The pathogenesis of cleft lip and palate (CL/P) is studied in animal experiments. 
      This study revealed significant differences in foetal secondary palate 
      development in two strains of mice (NMRI, A/WySnJ) using a palatal organ model. 
      Palatal shelves of 114 NMRI embryos, resistant to cleft occurrence, and 93 
      A/WySnJ embryos, a strain with a high spontaneous CL/P rate, were micro-dissected 
      at 14.25 GD (gestational day), before palatal fusion takes place. After 
      cultivation in serum-free medium, palatal development was investigated 
      microscopically and scored in a six-step system. At death (14.25 GD) the palatal 
      shelves of the NMRI embryos (mean 3.5) were significant more developed than those 
      of A/WySnJ (mean 2.7; p=0.05). After incubation, 53% (60/114) NMRI and 14% 
      (13/93) A/WySnJ cultures had over two-thirds fusion to stage V-VI, therefore in 
      17% NMRI (19/114) and 1% A/WySnJ cultures (1/93) fusion was macroscopically 
      complete. 62% of the A/WySnJ cultures showed no significant development in vitro 
      (mean 2.84; p=0.094). There is a significant palatal development difference 
      between normally developed NMRI (mean 4.45, p=0.05) and CL/P appearance in 
      A/WySnJ mice (mean 2.84). Palatal development of both strains was significantly 
      delayed in organ culture (p=0.05). The A/WySnJ strain was more susceptible to 
      manipulation and vulnerable.
CI  - Copyright (c) 2011 International Association of Oral and Maxillofacial Surgeons. 
      Published by Elsevier Ltd. All rights reserved.
FAU - Scheller, K
AU  - Scheller K
AD  - Department of Oral and Maxillofacial Plastic Surgery, Martin-Luther-University 
      Halle-Wittenberg, Germany. konstanze.weinzierl@medizin.uni-halle.de742 Scheller 
      et al.
FAU - Schubert, A
AU  - Schubert A
FAU - Schubert, J
AU  - Schubert J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110331
PL  - Denmark
TA  - Int J Oral Maxillofac Surg
JT  - International journal of oral and maxillofacial surgery
JID - 8605826
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - Animals
MH  - Cleft Lip/embryology
MH  - Cleft Palate/embryology
MH  - Culture Media, Serum-Free
MH  - Disease Models, Animal
MH  - Epithelium/embryology
MH  - Gestational Age
MH  - Mesoderm/embryology
MH  - Mice
MH  - Mice, Inbred A
MH  - Mice, Inbred Strains
MH  - Microdissection
MH  - Organ Culture Techniques
MH  - Organogenesis/*physiology
MH  - Palate/*embryology
EDAT- 2011/04/05 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/04/05 06:00
PHST- 2009/08/05 00:00 [received]
PHST- 2011/01/12 00:00 [revised]
PHST- 2011/02/10 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0901-5027(11)00095-6 [pii]
AID - 10.1016/j.ijom.2011.02.020 [doi]
PST - ppublish
SO  - Int J Oral Maxillofac Surg. 2011 Jul;40(7):737-42. doi: 
      10.1016/j.ijom.2011.02.020. Epub 2011 Mar 31.