PMID- 21458510
OWN - NLM
STAT- MEDLINE
DCOM- 20110908
LR  - 20181201
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 158
IP  - 1-2
DP  - 2011 Jun
TI  - Identification of immunodominant T-cell epitopes in membrane protein of highly 
      pathogenic porcine reproductive and respiratory syndrome virus.
PG  - 108-15
LID - 10.1016/j.virusres.2011.03.018 [doi]
AB  - The development of cell-mediated immunity has been known extremely important in 
      clearing porcine reproductive and respiratory syndrome virus (PRRSV) in infected 
      pigs. However, the PRRS immunology regarding the interaction of T-cells and PRRSV 
      proteins is poorly understood. To identify the T-cell immunodominant epitopes on 
      the membrane (M) protein of PRRSV, a series of 31 overlapping pentadecapeptides 
      covering the entire M protein were designed and synthesized. These peptides were 
      screened by ELIspot analysis for their capabilities to elicit interferon-gamma 
      (IFN-gamma) responses in the peripheral blood mononuclear cells (PBMCs), which were 
      collected from pigs immunized with attenuated PRRSV HuN4-F112 strain and 
      challenged with highly pathogenic HuN4 strain. After three rounds of screening, 4 
      peptides (M3, M6, M8 and M12) were shown to elicit high expression of IFN-gamma. The 
      stimulation of high IFN-gamma transcription in PBMCs by these 4 peptides was further 
      confirmed in real-time PCR. The sequence alignment revealed that the epitope 
      represented by peptide M6 was fully conserved in all of examined 42 North 
      American genotype II PRRSV isolates and the epitopes represented by peptides M3, 
      M8 and M12 showed 2-4 amino acid replacements. The finding of 4 T-cell 
      immunodominant epitopes in the M protein of PRRSV will be beneficial to the 
      understanding of the development of cell-mediated immunity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Wang, Ya-Xin
AU  - Wang YX
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Li, Guo-Xin
AU  - Li GX
FAU - Zhang, Shan-Rui
AU  - Zhang SR
FAU - Jiang, Yi-Feng
AU  - Jiang YF
FAU - Xu, Ao-Tian
AU  - Xu AT
FAU - Yu, Hai
AU  - Yu H
FAU - Wang, Meng-Meng
AU  - Wang MM
FAU - Yan, Li-Ping
AU  - Yan LP
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110331
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Matrix Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunospot Assay
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Immunodominant Epitopes/*immunology
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Matrix Proteins/*immunology
EDAT- 2011/04/05 06:00
MHDA- 2011/09/09 06:00
CRDT- 2011/04/05 06:00
PHST- 2010/11/29 00:00 [received]
PHST- 2011/03/20 00:00 [revised]
PHST- 2011/03/22 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/09/09 06:00 [medline]
AID - S0168-1702(11)00111-0 [pii]
AID - 10.1016/j.virusres.2011.03.018 [doi]
PST - ppublish
SO  - Virus Res. 2011 Jun;158(1-2):108-15. doi: 10.1016/j.virusres.2011.03.018. Epub 
      2011 Mar 31.