PMID- 21458538
OWN - NLM
STAT- MEDLINE
DCOM- 20110826
LR  - 20211203
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 495
IP  - 3
DP  - 2011 May 20
TI  - Akt/mammalian target of rapamycin signaling pathway regulates neurite outgrowth 
      in cerebellar granule neurons stimulated by methylcobalamin.
PG  - 201-4
LID - 10.1016/j.neulet.2011.03.065 [doi]
AB  - Methylcobalamin (MeCbl), a vitamin B12 analog, promotes neurite outgrowth by 
      activating Akt in neurons. However, Akt is involved in many cellular functions, 
      and the downstream signal of Akt that promotes neurite outgrowth in neurons in 
      the presence of MeCbl remains obscure. Mammalian target of rapamycin (mTOR) is a 
      serine/threonine protein kinase that regulates multiple cellular functions 
      including neurite outgrowth. mTOR is regarded as important for the regeneration 
      of injured nerves. In this study, we examined the relationship between MeCbl and 
      mTOR activity and found that MeCbl increases mTOR activity via the activation of 
      Akt and promotes neurite outgrowth in cerebellar granule neurons via the 
      activation of mTOR.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Okada, Kiyoshi
AU  - Okada K
AD  - Department of Orthopaedics, Osaka University Graduate School of Medicine, 2-2 
      Yamadaoka, Suita, Osaka 565-0871, Japan. kokada2433@snow.ocn.ne.jp
FAU - Tanaka, Hiroyuki
AU  - Tanaka H
FAU - Temporin, Ko
AU  - Temporin K
FAU - Okamoto, Michio
AU  - Okamoto M
FAU - Kuroda, Yusuke
AU  - Kuroda Y
FAU - Moritomo, Hisao
AU  - Moritomo H
FAU - Murase, Tsuyoshi
AU  - Murase T
FAU - Yoshikawa, Hideki
AU  - Yoshikawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110330
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Morpholines)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - BR1SN1JS2W (mecobalamin)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - P6YC3EG204 (Vitamin B 12)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Cerebellum/*cytology
MH  - Chromones/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Morpholines/pharmacology
MH  - Neurites/*drug effects
MH  - Neurons/*cytology/drug effects
MH  - Oncogene Protein v-akt/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*drug effects
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Vitamin B 12/*analogs & derivatives/pharmacology
EDAT- 2011/04/05 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/04/05 06:00
PHST- 2010/12/24 00:00 [received]
PHST- 2011/03/15 00:00 [revised]
PHST- 2011/03/21 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - S0304-3940(11)00379-X [pii]
AID - 10.1016/j.neulet.2011.03.065 [doi]
PST - ppublish
SO  - Neurosci Lett. 2011 May 20;495(3):201-4. doi: 10.1016/j.neulet.2011.03.065. Epub 
      2011 Mar 30.