PMID- 21459437
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20191210
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 32
IP  - 21
DP  - 2011 Jul
TI  - The role of bFGF in down-regulating alpha-SMA expression of chondrogenically induced 
      BMSCs and preventing the shrinkage of BMSC engineered cartilage.
PG  - 4773-81
LID - 10.1016/j.biomaterials.2011.03.020 [doi]
AB  - Bone marrow stromal cells (BMSCs) have proved to be an ideal cell source for 
      cartilage regeneration. Our previous studies demonstrated that a 
      three-dimensional (3D) cartilage could be constructed successfully in vitro using 
      BMSCs and biodegradable scaffolds. However, an obvious shrinkage and deformation 
      was observed during in vitro chondrogenic induction. According to the 
      literatures, it can be speculated that the up-regulation of smooth muscle 
      actin-alpha (alpha-SMA) caused by transforming growth factor beta (TGFbeta) is one of 
      the leading reasons and that basic fibroblast growth factor (bFGF) could 
      antagonize the role of TGFbeta to down-regulate alpha-SMA expression and prevent the 
      shrinkage of BMSC engineered cartilage. This study testified these speculations 
      by adding bFGF to chondrogenic media. According to the current results, 
      chondrogenic induction significantly up-regulated alpha-SMA expression of BMSCs at 
      both cell and tissue levels, and the engineered tissue only retained 12.4% of 
      original size after 6 weeks of chondrogenic induction. However, the supplement of 
      bFGF in chondrogenic media efficiently down-regulated alpha-SMA expression and the 
      engineered tissue still retained over 60% of original size after 6 weeks of 
      culture. Moreover, bFGF showed a beneficial influence on 3D cartilage formation 
      of BMSCs in terms of gene expression and deposition of cartilage specific 
      matrices. All these results suggested that bFGF could repress alpha-SMA expression 
      caused by chondrogenic induction, efficiently prevent shrinkage of BMSC 
      engineered tissue, and have a positive influence on cartilage formation, which 
      provides a clue for both shape control and quality improvement of BMSC engineered 
      3D cartilage.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Li, Qiong
AU  - Li Q
AD  - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, 
      Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of 
      Tissue Engineering, Shanghai Stem Cell Institute, 639 Zhi Zao Ju Road, Shanghai 
      200011, PR China.
FAU - Liu, Tianyi
AU  - Liu T
FAU - Zhang, Lu
AU  - Zhang L
FAU - Liu, Yu
AU  - Liu Y
FAU - Zhang, Wenjie
AU  - Zhang W
FAU - Liu, Wei
AU  - Liu W
FAU - Cao, Yilin
AU  - Cao Y
FAU - Zhou, Guangdong
AU  - Zhou G
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110402
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Actins)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Culture Media)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Actins/genetics/*metabolism
MH  - Animals
MH  - Biocompatible Materials/chemistry/metabolism
MH  - Bone Marrow Cells/cytology/*drug effects/*physiology
MH  - Cartilage/*cytology
MH  - Cell Culture Techniques
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chondrogenesis/drug effects
MH  - Culture Media/chemistry
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Materials Testing
MH  - Stromal Cells/cytology/*drug effects/*physiology
MH  - Swine
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/chemistry
EDAT- 2011/04/05 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/04/05 06:00
PHST- 2011/02/20 00:00 [received]
PHST- 2011/03/06 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - S0142-9612(11)00262-6 [pii]
AID - 10.1016/j.biomaterials.2011.03.020 [doi]
PST - ppublish
SO  - Biomaterials. 2011 Jul;32(21):4773-81. doi: 10.1016/j.biomaterials.2011.03.020. 
      Epub 2011 Apr 2.