PMID- 21460823
OWN - NLM
STAT- MEDLINE
DCOM- 20110701
LR  - 20240104
IS  - 1548-7105 (Electronic)
IS  - 1548-7091 (Linking)
VI  - 8
IP  - 5
DP  - 2011 May
TI  - A more efficient method to generate integration-free human iPS cells.
PG  - 409-12
LID - 10.1038/nmeth.1591 [doi]
AB  - We report a simple method, using p53 suppression and nontransforming L-Myc, to 
      generate human induced pluripotent stem cells (iPSCs) with episomal plasmid 
      vectors. We generated human iPSCs from multiple donors, including two putative 
      human leukocyte antigen (HLA)-homozygous donors who match  approximately 20% of the Japanese 
      population at major HLA loci; most iPSCs are integrated transgene-free. This 
      method may provide iPSCs suitable for autologous and allologous stem-cell therapy 
      in the future.
FAU - Okita, Keisuke
AU  - Okita K
AD  - Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. 
      okita@cira.kyoto-u.ac.jp
FAU - Matsumura, Yasuko
AU  - Matsumura Y
FAU - Sato, Yoshiko
AU  - Sato Y
FAU - Okada, Aki
AU  - Okada A
FAU - Morizane, Asuka
AU  - Morizane A
FAU - Okamoto, Satoshi
AU  - Okamoto S
FAU - Hong, Hyenjong
AU  - Hong H
FAU - Nakagawa, Masato
AU  - Nakagawa M
FAU - Tanabe, Koji
AU  - Tanabe K
FAU - Tezuka, Ken-ichi
AU  - Tezuka K
FAU - Shibata, Toshiyuki
AU  - Shibata T
FAU - Kunisada, Takahiro
AU  - Kunisada T
FAU - Takahashi, Masayo
AU  - Takahashi M
FAU - Takahashi, Jun
AU  - Takahashi J
FAU - Saji, Hiroh
AU  - Saji H
FAU - Yamanaka, Shinya
AU  - Yamanaka S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110403
PL  - United States
TA  - Nat Methods
JT  - Nature methods
JID - 101215604
RN  - 0 (HLA Antigens)
SB  - IM
CIN - Nat Methods. 2011 May;8(5):389-90. PMID: 21527929
MH  - Asian People/genetics
MH  - Cell Culture Techniques/*methods
MH  - Electroporation
MH  - Gene Expression Profiling
MH  - Gene Frequency
MH  - Genetic Vectors
MH  - HLA Antigens/genetics
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology/immunology/metabolism
MH  - Plasmids/genetics
MH  - Tissue Donors
EDAT- 2011/04/05 06:00
MHDA- 2011/07/02 06:00
CRDT- 2011/04/05 06:00
PHST- 2010/10/29 00:00 [received]
PHST- 2011/02/08 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/07/02 06:00 [medline]
AID - nmeth.1591 [pii]
AID - 10.1038/nmeth.1591 [doi]
PST - ppublish
SO  - Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.