PMID- 21461236
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Print)
IS  - 1226-4512 (Linking)
VI  - 15
IP  - 1
DP  - 2011 Feb
TI  - Quercetin Inhibits alpha3beta4 Nicotinic Acetylcholine Receptor-Mediated Ion Currents 
      Expressed in Xenopus Oocytes.
PG  - 17-22
LID - 10.4196/kjpp.2011.15.1.17 [doi]
AB  - Quercetin mainly exists in the skin of colored fruits and vegetables as one of 
      flavonoids. Recent studies show that quercetin, like other flavonoids, has 
      diverse pharmacological actions. However, relatively little is known about 
      quercetin effects in the regulations of ligand-gated ion channels. In the 
      previous reports, we have shown that quercetin regulates subsets of homomeric 
      ligand-gated ion channels such as glycine, 5-HT(3A) and alpha7 nicotinic 
      acetylcholine receptors. In the present study, we examined quercetin effects on 
      heteromeric neuronal alpha3beta4 nicotinic acetylcholine receptor channel activity 
      expressed in Xenopus oocytes after injection of cRNA encoding bovine neuronal alpha3 
      and beta4 subunits. Treatment with acetylcholine elicited an inward peak current 
      (I(ACh)) in oocytes expressing alpha3beta4 nicotinic acetylcholine receptor. 
      Co-treatment with quercetin and acetylcholine inhibited I(ACh) in oocytes 
      expressing alpha3beta4 nicotinic acetylcholine receptors. The inhibition of I(ACh) by 
      quercetin was reversible and concentration-dependent. The half-inhibitory 
      concentration (IC(50)) of quercetin was 14.9+/-0.8 microM in oocytes expressing alpha3beta4 
      nicotinic acetylcholine receptor. The inhibition of I(ACh) by quercetin was 
      voltage-independent and non-competitive. These results indicate that quercetin 
      might regulate alpha3beta4 nicotinic acetylcholine receptor and this regulation might be 
      one of the pharmacological actions of quercetin in nervous systems.
FAU - Lee, Byung-Hwan
AU  - Lee BH
AD  - Department of Physiology, College of Veterinary Medicine and Bio/Molecular 
      Informatics Center, Konkuk University, Seoul 143-701, Korea.
FAU - Hwang, Sung-Hee
AU  - Hwang SH
FAU - Choi, Sun-Hye
AU  - Choi SH
FAU - Shin, Tae-Joon
AU  - Shin TJ
FAU - Kang, Jiyeon
AU  - Kang J
FAU - Lee, Sang-Mok
AU  - Lee SM
FAU - Nah, Seung-Yeol
AU  - Nah SY
LA  - eng
PT  - Journal Article
DEP - 20110228
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC3062079
OTO - NOTNLM
OT  - Flavonoids
OT  - Quercetin
OT  - Xenopus oocyte
OT  - alpha3beta4 nicotinic acetylcholine receptor
EDAT- 2011/04/05 06:00
MHDA- 2011/04/05 06:01
PMCR- 2011/02/01
CRDT- 2011/04/05 06:00
PHST- 2010/12/27 00:00 [received]
PHST- 2011/01/17 00:00 [revised]
PHST- 2011/01/18 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/04/05 06:01 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2011.15.1.17 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2011 Feb;15(1):17-22. doi: 10.4196/kjpp.2011.15.1.17. 
      Epub 2011 Feb 28.