PMID- 21461978
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR  - 20211020
IS  - 1433-4909 (Electronic)
IS  - 1431-0651 (Linking)
VI  - 15
IP  - 3
DP  - 2011 May
TI  - Response to adverse conditions in two strains of the extremely halophilic species 
      Salinibacter ruber.
PG  - 379-89
LID - 10.1007/s00792-011-0366-3 [doi]
AB  - We have studied the response of the two closest relative strains M8 and M31 of 
      Salinibacter ruber to environmental changes as the transition from exponential to 
      stationary phase in a batch growth, and the submission to two different 
      environmental stresses (dilution of the culture medium and temperature decrease). 
      We monitored the changes in cultivability, ribosomal content by fluorescence in 
      situ hybridization (FISH), and metabolic changes with high-field ion cyclotron 
      Fourier transform mass spectrometry. In all cases, we could observe an important 
      decrease in cultivability that was not accompanied by a decrease in FISH counts, 
      pointing to a transition to viable but non-cultivable state rather than cell 
      death. Furthermore, the metabolomic analyses indicated a common response of both 
      strains to the different conditions assayed. Only a small portion of the detected 
      masses could be annotated due to database constraints. Among them, the most 
      remarkable changes could be attributed to modifications in the composition of the 
      cell envelope, and especially in the cell membrane. We could track changes in the 
      length or saturation of the fatty acids and in the composition of phospholipids 
      involved in aminosugar, glycerolipid, and glycerophospholipid metabolic pathways.
FAU - Brito-Echeverria, Jocelyn
AU  - Brito-Echeverria J
AD  - Marine Microbiology Group, Institut Mediterrani d' Estudis Avancats (CSIC-UIB), 
      07190, Esporles, Spain. jocelyn.brito@uib.es
FAU - Lucio, Marianna
AU  - Lucio M
FAU - Lopez-Lopez, Arantxa
AU  - Lopez-Lopez A
FAU - Anton, Josefa
AU  - Anton J
FAU - Schmitt-Kopplin, Philippe
AU  - Schmitt-Kopplin P
FAU - Rossello-Mora, Ramon
AU  - Rossello-Mora R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110403
PL  - Germany
TA  - Extremophiles
JT  - Extremophiles : life under extreme conditions
JID - 9706854
RN  - 0 (Fatty Acids)
RN  - 0 (Glycerophospholipids)
RN  - 0 (Phospholipids)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Energy Metabolism
MH  - Fatty Acids/metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Glycerophospholipids/metabolism
MH  - Halobacteriales/classification/genetics/growth & development/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Mass Spectrometry
MH  - Metabolomics/methods
MH  - Phospholipids/metabolism
MH  - Ribosomes/metabolism
MH  - Salinity
MH  - *Stress, Physiological
MH  - Temperature
EDAT- 2011/04/05 06:00
MHDA- 2011/08/24 06:00
CRDT- 2011/04/05 06:00
PHST- 2010/12/05 00:00 [received]
PHST- 2011/03/16 00:00 [accepted]
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - 10.1007/s00792-011-0366-3 [doi]
PST - ppublish
SO  - Extremophiles. 2011 May;15(3):379-89. doi: 10.1007/s00792-011-0366-3. Epub 2011 
      Apr 3.