PMID- 21462140
OWN - NLM
STAT- MEDLINE
DCOM- 20110531
LR  - 20131121
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 2
DP  - 2011 Apr
TI  - [Comparison and significance of DCDF probe and ES probe in the detection of 
      BCR/ABL fusion gene].
PG  - 220-2
LID - 10.3760/cma.j.issn.1003-9406.2011.02.022 [doi]
AB  - OBJECTIVE: To investigate the signal patterns of dual color dual fusion (DCDF) 
      probe and extra signal (ES) probe in the detection of BCR/ABL fusion gene, and 
      illustrate the relation between the fluorescence in situ hybridization (FISH) 
      pattern and the karyotype. METHODS: Sixty-five cases of chronic myelocytic 
      leukemia (CML) and 50 cases of acute lymphoblastic leukemia (ALL) were detected 
      by FISH with DCDF probe, the BCR/ABL positive samples were detected by FISH with 
      ES probe. Among these cases, 47 cases of CML and 40 cases of ALL perform 
      conventional cytogenetics simultaneously. RESULTS: All 65 cases of CML were all 
      BCR/ABL positive by FISH. 17 cases showed the atypical pattern by DCDF-FISH, and 
      12 cases showed the atypical pattern by ES-FISH. There were 7 cases of BCR/ABL 
      positive in 50 cases of ALL by FISH. By ES-FISH, there were 5 cases in which the 
      break-point of BCR gene was located in m-bcr, 2 cases in which the break-point of 
      BCR gene was located in M-bcr. Conventional cytogenetics demonstrated that 
      43/44(98%) cases of CML and 7/32(22%) cases of ALL were Ph positive. CONCLUSION: 
      The features of DCDF-FISH, ES-FISH and conventional cytogenetic are different 
      from each other. According to the features of these method, it can increase the 
      precision of the adjustment of genetic feature to analyze these results 
      comprehensively.
FAU - DU, Qing-hua
AU  - DU QH
AD  - Department of Hematology, Guangzhou First Municipal People's Hospital, Guangzhou, 
      Guangdong, 510180 P. R. China. du_haven@yahoo.com.cn
FAU - YING, Yi
AU  - YING Y
FAU - CHEN, Xiao-yan
AU  - CHEN XY
FAU - LI, Qing-shan
AU  - LI QS
FAU - WANG, Shun-qing
AU  - WANG SQ
FAU - MO, Wen-jian
AU  - MO WJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Cytogenetics/methods
MH  - *DNA Probes
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics
EDAT- 2011/04/05 06:00
MHDA- 2011/06/01 06:00
CRDT- 2011/04/05 06:00
PHST- 2011/04/05 06:00 [entrez]
PHST- 2011/04/05 06:00 [pubmed]
PHST- 2011/06/01 06:00 [medline]
AID - 940628045 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.02.022 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Apr;28(2):220-2. doi: 
      10.3760/cma.j.issn.1003-9406.2011.02.022.