PMID- 21463644
OWN - NLM
STAT- MEDLINE
DCOM- 20111014
LR  - 20211020
IS  - 1873-507X (Electronic)
IS  - 0031-9384 (Print)
IS  - 0031-9384 (Linking)
VI  - 104
IP  - 2
DP  - 2011 Aug 3
TI  - Histone deacetylase 1 (HDAC1) participates in the down-regulation of 
      corticotropin releasing hormone gene (crh) expression.
PG  - 312-20
LID - 10.1016/j.physbeh.2011.03.026 [doi]
AB  - The paraventricular nucleus of the hypothalamus (PVH) plays a central role in 
      regulating the hypothalamic-pituitary-adrenal (HPA) axis. Medial parvocellular 
      neurons of the PVH (mpPVH) integrate sensory and humoral inputs to maintain 
      homeostasis. Humoral inputs include glucocorticoids secreted by the adrenals, 
      which down-regulate HPA activation. A primary glucocorticoid target is the 
      population of mpPVH neurons that synthesize and secrete corticotropin-releasing 
      factors, the most potent of which is corticotropin-releasing hormone (CRH). 
      Although CRH gene (crh) expression is known to be down-regulated by 
      glucocorticoids, the mechanisms by which this process occurs are still poorly 
      understood. To begin this study we postulated that glucocorticoid repression of 
      crh involves HDAC recruitment to the region of the crh proximal promoter. To 
      evaluate this hypothesis, we treated hypothalamic cells that express CRH with the 
      HDAC inhibitor trichostatin A (TSA). As predicted, treatment with TSA led to 
      increased CRH mRNA levels and crh promoter activity. Although co-treatment with 
      Dex (10(-7)M) reduced the TSA effect on mRNA levels, it failed to reduce promoter 
      activity; however co-transfection of HDAC1 but not 3 restored Dex inhibition. A 
      distinction between HDAC1 and 3 was also apparent with respect to crh promoter 
      occupancy. Dex led to increased HDAC1 but not HDAC3 occupancy. In vivo studies 
      revealed that CRH-immunoreactive (-ir) neurons contained HDAC1- and HDAC3-ir. 
      Collectively, these data point to a role for HDAC1 in the physiologic regulation 
      of crh.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Miller, Lydia
AU  - Miller L
AD  - Department of Pathology, University of Virginia, 415 Lane Road, Charlottesville, 
      VA 22908, USA.
FAU - Foradori, Chad D
AU  - Foradori CD
FAU - Lalmansingh, Avin S
AU  - Lalmansingh AS
FAU - Sharma, Dharmendra
AU  - Sharma D
FAU - Handa, Robert J
AU  - Handa RJ
FAU - Uht, Rosalie M
AU  - Uht RM
LA  - eng
GR  - R01 MH082900/MH/NIMH NIH HHS/United States
GR  - R01 MH082900-01A2/MH/NIMH NIH HHS/United States
GR  - R01 MH82900/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110401
PL  - United States
TA  - Physiol Behav
JT  - Physiology & behavior
JID - 0151504
RN  - 0 (Glucocorticoids)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (RNA, Messenger)
RN  - 3X2S926L3Z (trichostatin A)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - EC 3.5.1.98 (Histone Deacetylase 1)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Adrenalectomy
MH  - Analysis of Variance
MH  - Animals
MH  - Cell Line
MH  - Chromatin Immunoprecipitation
MH  - Corticotropin-Releasing Hormone/*genetics/metabolism
MH  - Dexamethasone/pharmacology
MH  - Down-Regulation/drug effects/*physiology
MH  - Drug Interactions
MH  - Glucocorticoids/pharmacology
MH  - Histone Deacetylase 1/*metabolism
MH  - Histone Deacetylase Inhibitors/pharmacology
MH  - Histone Deacetylases/genetics/metabolism
MH  - Hydroxamic Acids/pharmacology
MH  - Male
MH  - Paraventricular Hypothalamic Nucleus/drug effects/*enzymology
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC3650854
MID - NIHMS287312
EDAT- 2011/04/06 06:00
MHDA- 2011/10/15 06:00
PMCR- 2013/05/10
CRDT- 2011/04/06 06:00
PHST- 2011/01/13 00:00 [received]
PHST- 2011/03/10 00:00 [revised]
PHST- 2011/03/25 00:00 [accepted]
PHST- 2011/04/06 06:00 [entrez]
PHST- 2011/04/06 06:00 [pubmed]
PHST- 2011/10/15 06:00 [medline]
PHST- 2013/05/10 00:00 [pmc-release]
AID - S0031-9384(11)00145-4 [pii]
AID - 10.1016/j.physbeh.2011.03.026 [doi]
PST - ppublish
SO  - Physiol Behav. 2011 Aug 3;104(2):312-20. doi: 10.1016/j.physbeh.2011.03.026. Epub 
      2011 Apr 1.