PMID- 21463659
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20110516
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 72
IP  - 6
DP  - 2011 Jun
TI  - Short tandem repeat and human leukocyte antigen mutations or losses confound 
      engraftment and typing analysis in hematopoietic stem cell transplants.
PG  - 503-9
LID - 10.1016/j.humimm.2011.03.003 [doi]
AB  - Clonal chromosomal abnormalities are often found in the tumor cells of patients 
      with malignancies. These abnormalities can cause downregulation of human 
      leukocyte antigen (HLA) and instability of short tandem repeat (STR) DNA 
      sequences, confounding HLA typing and/or engraftment analysis in hematopoietic 
      stem cell transplants (HSCT). We describe here the abnormalities observed during 
      testing of 600 HSCT patients. HLA molecular typing was performed by reference 
      strand conformational analyses and/or sequence-based typing. STR testing was 
      performed with 10 to 16 STR primer sets, following 1 to 4 informative loci in 
      each patient. Eight patients exhibited either loss of heterozygosity (4 STR, 3 
      HLA) or STR length mutation (n = 1), and 5 of the 8 exhibited correlative 
      cytogenetic abnormalities. Diagnoses were acute myelogenous leukemia (AML; n = 7) 
      or myelofibrosis (MFIB: n = 1), yielding an 11% incidence of these chromosomal 
      abnormalities among the subset of 72 AML/MFIB HSCT patients. These results 
      highlight some of the problems encountered and the possibility for interpretive 
      errors that can arise when analyzing molecular typing and engraftment data, 
      particularly among AML/MFIB patients.
CI  - Copyright (c) 2011. Published by Elsevier Inc.
FAU - Pereira, Shalini
AU  - Pereira S
AD  - Clinical Research Division, Department of Laboratory Medicine, University of 
      Washington, Seattle, WA 98109, USA. spereira@seattlecca.org
FAU - Vayntrub, Tamara
AU  - Vayntrub T
FAU - Hiraki, Debra D
AU  - Hiraki DD
FAU - Cherry, Athena M
AU  - Cherry AM
FAU - Arai, Sally
AU  - Arai S
FAU - Dvorak, Christopher C
AU  - Dvorak CC
FAU - Grumet, F Carl
AU  - Grumet FC
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20110402
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Chromosome Aberrations/statistics & numerical data
MH  - DNA Mutational Analysis/methods
MH  - Female
MH  - HLA Antigens/*genetics
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing/methods
MH  - Humans
MH  - Incidence
MH  - Leukemia, Myeloid, Acute/diagnosis/epidemiology/*genetics/immunology
MH  - Male
MH  - Microsatellite Repeats/*genetics
MH  - Mutation/genetics
MH  - Primary Myelofibrosis/diagnosis/epidemiology/*genetics/immunology
MH  - Transplantation Tolerance/genetics
EDAT- 2011/04/06 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/04/06 06:00
PHST- 2010/08/06 00:00 [received]
PHST- 2011/02/16 00:00 [revised]
PHST- 2011/03/09 00:00 [accepted]
PHST- 2011/04/06 06:00 [entrez]
PHST- 2011/04/06 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - S0198-8859(11)00056-5 [pii]
AID - 10.1016/j.humimm.2011.03.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2011 Jun;72(6):503-9. doi: 10.1016/j.humimm.2011.03.003. Epub 2011 
      Apr 2.