PMID- 21467633
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 34
IP  - 4
DP  - 2011
TI  - Transforming growth factor beta1 promotes migration of human periodontal ligament 
      cells through heat shock protein 27 phosphorylation.
PG  - 486-9
AB  - Transforming growth factor beta1 (TGF-beta1) regulates cellular functions including 
      cell growth, differentiation, and migration. However, signal transduction 
      pathways of TGF-beta1 are mostly undefined in human periodontal ligament (hPDL) 
      cells. In this study, we investigated TGF beta1-induced migration focusing on heat 
      shock protein 27 (Hsp27) activation. The cellular response to TGF-beta1 by hPDL 
      cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium 
      bromide (MTT) assay and Western blot analysis. Cell migration was determined by 
      counting migrated cells using a Chemotaxis Cell Migration Assay kit. TGF-beta1 
      induced cell migration and increased the phosphorylation of Hsp27 and p38 
      mitogen-activated protein kinase (MAPK) in hPDL cells. Inhibiting p38 MAPK with 
      SB203580 blocked TGF-beta1-induced Hsp27 activation and cell migration. These 
      findings suggest that TGF-beta1-induced Hsp27 may promote the migration of hPDL 
      cells via p38 MAPK mediated signaling. Hsp27 may be a potential target for the 
      periodontal regeneration process related to cell migration.
FAU - Kwon, Seong-Min
AU  - Kwon SM
AD  - Department of Pathology, Chosun University, School of Dentistry, Seoseok-dong, 
      Dong-gu, Gwangju, Korea.
FAU - Kim, Soo-A
AU  - Kim SA
FAU - Fujii, Shinsuke
AU  - Fujii S
FAU - Maeda, Hidefumi
AU  - Maeda H
FAU - Ahn, Sang-Gun
AU  - Ahn SG
FAU - Yoon, Jung-Hoon
AU  - Yoon JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - *Cell Movement
MH  - HSP27 Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Periodontal Ligament/*cytology/physiology
MH  - Phosphorylation
MH  - Pyridines/pharmacology
MH  - Signal Transduction
MH  - Transforming Growth Factor beta1/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2011/04/07 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/04/07 06:00
PHST- 2011/04/07 06:00 [entrez]
PHST- 2011/04/07 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - JST.JSTAGE/bpb/34.486 [pii]
AID - 10.1248/bpb.34.486 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2011;34(4):486-9. doi: 10.1248/bpb.34.486.