PMID- 21467828
OWN - NLM
STAT- MEDLINE
DCOM- 20110824
LR  - 20190727
IS  - 1349-3329 (Electronic)
IS  - 0040-8727 (Linking)
VI  - 223
IP  - 4
DP  - 2011 Apr
TI  - Terahertz-wave spectroscopy for precise histopathological imaging of tumor and 
      non-tumor lesions in paraffin sections.
PG  - 291-6
AB  - Terahertz (THz; 10(12) Hz) waves have a frequency from 0.1 to 10 THz between the 
      visible light and microwave domains. THz waves are expected to be useful for 
      analysis of the histological features, without any staining procedure that is an 
      indispensable prerequisite for optical microscopy. It has been demonstrated that 
      THz transmittances at cancer and normal tissues are different. However, 
      spectroscopy that is currently used is applicable for imaging only small areas at 
      fixed-wavelength. In this study, we have developed a spectrometer employing a 
      gallium phosphide (GaP) THz-generator and applied it to examine large areas of 
      tissue specimens using a wide range of wavelengths. We thus examined the whole 
      areas of two paraffin sections (metastatic liver cancer and acute myocardial 
      infarction) in a frequency range of 1 to 6 THz, and compared the THz images of 
      ordinary paraffin sections with the histological features detected by microscopy. 
      THz imaging showed striking contrasts between cancerous and non-cancerous regions 
      at 3.7 THz. Likewise, the precise imaging was achieved in the infarct myocardium 
      at 3.6 THz. Images of THz transmittances in optimal wavelength were well matched 
      with HE histological features both in cancer and myocardial tissues. Cancer 
      regions showed higher transmittance than non-cancerous regions in liver. Old scar 
      regions showed low transmittance, and necrotic regions showed relatively higher 
      transmittance than normal myocardial areas. Thus, THz imaging precisely reflects 
      tissue conditions such as tumor, non-tumor tissues, tissue degeneration and 
      fibrosis. The newly established THz spectroscopy would be useful for pathological 
      diagnosis of routinely processed specimens.
FAU - Miura, Yasuhiro
AU  - Miura Y
AD  - Department of Pathology, Iwate Medical University School of Medicine, Morioka, 
      Japan.
FAU - Kamataki, Akihisa
AU  - Kamataki A
FAU - Uzuki, Miwa
AU  - Uzuki M
FAU - Sasaki, Tetsuo
AU  - Sasaki T
FAU - Nishizawa, Jun-ichi
AU  - Nishizawa J
FAU - Sawai, Takashi
AU  - Sawai T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
SB  - IM
MH  - Diagnostic Imaging
MH  - Humans
MH  - Liver Neoplasms/*pathology
MH  - Myocardial Infarction/*pathology
MH  - *Paraffin Embedding
MH  - Terahertz Spectroscopy/*methods
EDAT- 2011/04/07 06:00
MHDA- 2011/08/25 06:00
CRDT- 2011/04/07 06:00
PHST- 2011/04/07 06:00 [entrez]
PHST- 2011/04/07 06:00 [pubmed]
PHST- 2011/08/25 06:00 [medline]
AID - JST.JSTAGE/tjem/223.291 [pii]
AID - 10.1620/tjem.223.291 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 2011 Apr;223(4):291-6. doi: 10.1620/tjem.223.291.