PMID- 21468608
OWN - NLM
STAT- MEDLINE
DCOM- 20110713
LR  - 20200310
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 4
IP  - 3
DP  - 2011 May-Jun
TI  - Smad2 and Smad3 as mediators of the response of adventitial fibroblasts induced 
      by transforming growth factor beta1.
PG  - 561-7
LID - 10.3892/mmr.2011.458 [doi]
AB  - Transforming growth factor beta1 (TGF-beta1) is a known pleiotropic growth factor in 
      cell proliferation, migration and phenotypic transition. Fibroblasts are 
      considered the most reactive in the vascular wall. We aimed to explore the effect 
      and mechanism of TGF-beta1 on aortal adventitial fibroblasts (AFs) induced by 
      TGF-beta1. AFs were cultured in vitro by tissue explants. After stimulation by 
      TGF-beta1 and treatment with small interfering RNA (siRNA)-Smad2 and siRNA-Smad3, 
      MTT and the transwell chamber techniques were used for assessing AF proliferation 
      and migration. The expression of phospho (pho)-Smad2, pho-Smad3, Smad7, alpha-smooth 
      muscle actin (SMA) and collagen I and III were evaluated by Western blot analysis 
      and real-time RT-PCR. After stimulation by TGF-beta1 for 24 h, the proliferation and 
      migration of treated AFs were higher compared to those of untreated AFs, as was 
      the expression of Smad2, Smad3, pho-Smad2, pho-Smad3, SMA and collagen I and III 
      (P<0.05), but not Smad7 (P>0.05). Knockdown of Smad2 and Smad3 inhibited 
      proliferation and migration of AFs and down-regulated the expression of SMA and 
      collagen I and III (P<0.05). TGF-beta1 plays a key role in remodeling processes by 
      contributing to the proliferation, migration and phenotypic modulation of AFs and 
      collagen composition. The mechanism may be related to both the Smad2 and Smad3 
      signaling pathways.
FAU - Ren, Min
AU  - Ren M
AD  - Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, 
      Jinan, Shandong 250012, PR China.
FAU - Wang, Bo
AU  - Wang B
FAU - Zhang, Jidong
AU  - Zhang J
FAU - Liu, Ping
AU  - Liu P
FAU - Lv, Yijing
AU  - Lv Y
FAU - Liu, Guilin
AU  - Liu G
FAU - Jiang, Hong
AU  - Jiang H
FAU - Liu, Fenye
AU  - Liu F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110318
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad2 protein, rat)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Transdifferentiation/drug effects
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Fibroblasts/cytology/*drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Smad2 Protein/genetics/*metabolism
MH  - Smad3 Protein/genetics/*metabolism
MH  - Transforming Growth Factor beta1/*pharmacology
EDAT- 2011/04/07 06:00
MHDA- 2011/07/14 06:00
CRDT- 2011/04/07 06:00
PHST- 2010/11/03 00:00 [received]
PHST- 2011/03/03 00:00 [accepted]
PHST- 2011/04/07 06:00 [entrez]
PHST- 2011/04/07 06:00 [pubmed]
PHST- 2011/07/14 06:00 [medline]
AID - 10.3892/mmr.2011.458 [doi]
PST - ppublish
SO  - Mol Med Rep. 2011 May-Jun;4(3):561-7. doi: 10.3892/mmr.2011.458. Epub 2011 Mar 
      18.