PMID- 21468941
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20211020
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 728
DP  - 2011
TI  - Intact-protein analysis system for discovery of serum-based disease biomarkers.
PG  - 69-85
LID - 10.1007/978-1-61779-068-3_4 [doi]
AB  - Profiling of serum and plasma proteins has substantial relevance to the discovery 
      of circulating disease biomarkers. However, the extreme complexity and vast 
      dynamic range of protein abundance in serum and plasma present a formidable 
      challenge for protein analysis. Thus, integration of multiple technologies is 
      required to achieve high-resolution and high-sensitivity proteomic analysis of 
      serum or plasma. In this chapter, we describe an orthogonal multidimensional 
      intact-protein analysis system (IPAS) (Wang et al., Mol Cell Proteomics 
      4:618-625, 2005) coupled with protein tagging (Faca et al., J Proteome Res 
      5:2009-2018, 2006) to profile the serum and plasma proteomes quantitatively, 
      which we have applied in our biomarker discovery studies (Katayama et al., Genome 
      Med 1:47, 2009; Faca et al., PLoS Med 5:e123, 2008; Zhang et al. Genome Biol 
      9:R93, 2008).
FAU - Wang, Hong
AU  - Wang H
AD  - Fred Hutchinson Cancer Research Center, North Seattle, WA, USA.
FAU - Hanash, Samir
AU  - Hanash S
LA  - eng
GR  - U01 CA084982/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Anions)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Proteins)
SB  - IM
MH  - Anions
MH  - Biomarkers/blood
MH  - Blood Proteins/*analysis
MH  - Chemical Fractionation
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Ion Exchange
MH  - Chromatography, Reverse-Phase
MH  - *Disease
MH  - Humans
MH  - Isotope Labeling
MH  - Nanotechnology
MH  - Proteomics/*methods
MH  - Serum/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Statistics as Topic
MH  - Temperature
PMC - PMC4677997
MID - NIHMS742211
EDAT- 2011/04/07 06:00
MHDA- 2011/07/13 06:00
PMCR- 2015/12/14
CRDT- 2011/04/07 06:00
PHST- 2011/04/07 06:00 [entrez]
PHST- 2011/04/07 06:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
PHST- 2015/12/14 00:00 [pmc-release]
AID - 10.1007/978-1-61779-068-3_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;728:69-85. doi: 10.1007/978-1-61779-068-3_4.