PMID- 21469111
OWN - NLM
STAT- MEDLINE
DCOM- 20110624
LR  - 20211020
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 41
IP  - 5
DP  - 2011 May
TI  - Translocation of an antibody transgene requires AID and occurs by 
      interchromosomal switching to all switch regions except the mu switch region.
PG  - 1456-64
LID - 10.1002/eji.201041077 [doi]
AB  - Immunoglobulin (Ig) class switch recombination (CSR) occurs most often by 
      intrachromosomal recombinations between switch (S) regions located on a single 
      chromosome, but it can also occur by interchomosomal recombinations between Ig 
      heavy chain (Igh) S regions located on chomosomal homologs. Interchromosomal 
      recombinations have also been found between chromosomes that are not homologs; 
      examples are Igh/c-myc and Igh/transgene translocations. Most, but not all, 
      studies have indicated that activation-induced cytidine deaminase (AID) is 
      important in Igh/c-myc translocations. The role of AID has not been determined 
      for Igh/transgene translocations. We now show that the majority of Igh/transgene 
      translocations between non-homologs from an Ig transgenic mouse are dependent on 
      AID, but we also find a small number of these translocations that can occur in 
      the absence of AID. Surprisingly, our results also indicate that, although Sgamma 
      switch sequences in the endogenous Igh locus participate in chromosomal 
      translocations with the non-homolog transgene-bearing chromosome, Smu switch 
      sequences do not. This contrasts with the fact that both endogenous Smu and Sgamma 
      sequences participate in intrachromosomal CSR. Our findings suggest the operation 
      of a regulatory mechanism that can differentially control the accessibility of Smu 
      and Sgamma regions for non-homolog translocations even when both are accessible for 
      intrachromosomal recombination.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Shansab, Maryam
AU  - Shansab M
AD  - Program in Immunology and Department of Pathology, Sackler School of Graduate 
      Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA.
FAU - Eccleston, Jennifer M
AU  - Eccleston JM
FAU - Selsing, Erik
AU  - Selsing E
LA  - eng
GR  - R01 AI024465/AI/NIAID NIH HHS/United States
GR  - R01 AI024465-15/AI/NIAID NIH HHS/United States
GR  - AI24465/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110411
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - EC 3.5.4.- (AICDA (activation-induced cytidine deaminase))
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cytidine Deaminase/deficiency/genetics/*metabolism
MH  - *Genes, Immunoglobulin Heavy Chain
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Switch Region/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Transgenic
MH  - Polymerase Chain Reaction
MH  - Recombination, Genetic
MH  - *Transgenes
MH  - *Translocation, Genetic
PMC - PMC3142474
MID - NIHMS297859
COIS- Conflict of interest The authors have no financial conflict of interest.
EDAT- 2011/04/07 06:00
MHDA- 2011/06/28 06:00
PMCR- 2012/05/01
CRDT- 2011/04/07 06:00
PHST- 2010/09/20 00:00 [received]
PHST- 2011/01/20 00:00 [revised]
PHST- 2011/02/09 00:00 [accepted]
PHST- 2011/04/07 06:00 [entrez]
PHST- 2011/04/07 06:00 [pubmed]
PHST- 2011/06/28 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - 10.1002/eji.201041077 [doi]
PST - ppublish
SO  - Eur J Immunol. 2011 May;41(5):1456-64. doi: 10.1002/eji.201041077. Epub 2011 Apr 
      11.