PMID- 21470373
OWN - NLM
STAT- MEDLINE
DCOM- 20120126
LR  - 20110902
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 33
IP  - 5
DP  - 2011 Oct
TI  - Correlation of assessment of plasma cells by flow cytometry and detection of 
      cytogenomic abnormalities by fluorescence in situ hybridization in plasma cell 
      neoplasms.
PG  - 545-50
LID - 10.1111/j.1751-553X.2011.01323.x [doi]
AB  - INTRODUCTION: Increased monoclonal plasma cell count (PCC) in bone marrow (BM) is 
      an indicator of a plasma cell neoplasm (PCN). Assessing PCC in BM by morphologic 
      evaluation (ME) and flow cytometry (FC) is important in diagnosing PCN. Also 
      important is fluorescence in situ hybridization (FISH) to detect cytogenomic 
      abnormalities (CAs) in PCN. Owing to FC's short turnaround time, FC-assessed PCC 
      is often used as empirical reference for further FISH study. However, correlation 
      of FC-assessed PCs with FISH-detected CAs has not been well studied. The purpose 
      of this study was to validate this correlation on BM aspirates from patients with 
      PCN. METHODS: We reviewed 224 cases of newly diagnosed PCN identified from our 
      Lab-database. Both FC and FISH had been performed on BM aspirates from these 
      cases, of which 178 had been assessed by ME. The original findings from the 224 
      cases were also reviewed. RESULTS: Using a cut-off of 3% PCs assessed by FC as an 
      indicator for further FISH analysis, 20 (17%) FISH-detectable PCN cases were 
      missed; 30% of the missed cases carried high-risk CAs and/or were highly 
      progressive PCN. CONCLUSION: In PCN cases, an FC-assessed 3% PCC in BM aspirates 
      should not be used as a cut-off for further FISH testing.
CI  - (c) 2011 Blackwell Publishing Ltd.
FAU - Lu, G
AU  - Lu G
AD  - Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 
      Houston, TX 77030, USA. gglu@mdanderson.org
FAU - Zhang, X X
AU  - Zhang XX
FAU - You, M J
AU  - You MJ
FAU - Chen, W
AU  - Chen W
LA  - eng
PT  - Journal Article
DEP - 20110407
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
SB  - IM
MH  - Aged
MH  - Bone Marrow/metabolism/pathology
MH  - *Chromosome Aberrations
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*diagnosis/genetics/metabolism
EDAT- 2011/04/08 06:00
MHDA- 2012/01/27 06:00
CRDT- 2011/04/08 06:00
PHST- 2011/04/08 06:00 [entrez]
PHST- 2011/04/08 06:00 [pubmed]
PHST- 2012/01/27 06:00 [medline]
AID - 10.1111/j.1751-553X.2011.01323.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2011 Oct;33(5):545-50. doi: 10.1111/j.1751-553X.2011.01323.x. 
      Epub 2011 Apr 7.