PMID- 21470695
OWN - NLM
STAT- MEDLINE
DCOM- 20111026
LR  - 20110509
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 141
IP  - 3-4
DP  - 2011 Jun 15
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV)-specific mAbs: 
      supporting diagnostics and providing new insights into the antigenic properties 
      of the virus.
PG  - 246-57
LID - 10.1016/j.vetimm.2011.03.008 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the 
      most important viral pathogens in the swine industry. Despite great efforts of 
      pig holders, veterinarians, researchers and vaccine developers, the virus still 
      causes major production losses. It is clear that efficient and correct monitoring 
      and rational development of vaccines are crucial in the combat against this 
      pathogen. PRRSV-specific monoclonal antibodies (mAbs) are essential tools for 
      both diagnostic and research purposes. This study describes the production of 
      PRRSV GP3-, GP5- and N-specific hybridomas and an extensive characterization of 
      the mAbs. The N-specific mAbs generated in this study appear to be useful tools 
      for diagnostics, as they were found to react with genetically very different 
      PRRSV isolates and may serve to discriminate between European and American type 
      PRRSV isolates. These mAbs also allowed detection of the PRRSV N protein in both 
      formalin-fixed, paraffin-embedded tissue sections and frozen tissue sections of 
      PRRSV-infected lungs, further illustrating their diagnostic value. Different 
      neutralization assays pointed out that none of the GP3- and GP5-specific mAbs 
      tested shows virus-neutralizing capacity. This is noteworthy, as these mAbs 
      recognize epitopes in the predicted ectodomains of their target protein and since 
      the GP5-specific antibodies specifically react with the antigenic region that 
      corresponds to the "major neutralizing epitope" suggested for American type 
      PRRSV. The current findings argue against an important role of the identified 
      antigenic regions in direct antibody-mediated neutralization of European type 
      PRRSV in vivo. However, it is also clear that findings concerning a specific 
      PRRSV epitope cannot always be generalized, as the antigenic determinants and 
      their biological properties may differ radically between different virus 
      isolates.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Van Breedam, Wander
AU  - Van Breedam W
AD  - Department of Virology, Parasitology and Immunology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. 
      wander.vanbreedam@UGent.be
FAU - Costers, Sarah
AU  - Costers S
FAU - Vanhee, Merijn
AU  - Vanhee M
FAU - Gagnon, Carl A
AU  - Gagnon CA
FAU - Rodriguez-Gomez, Irene M
AU  - Rodriguez-Gomez IM
FAU - Geldhof, Marc
AU  - Geldhof M
FAU - Verbeeck, Mieke
AU  - Verbeeck M
FAU - Van Doorsselaere, Jan
AU  - Van Doorsselaere J
FAU - Karniychuk, Uladzimir
AU  - Karniychuk U
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110312
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Viral/*immunology
MH  - Cell Line
MH  - Epitopes
MH  - Gene Expression Regulation, Viral
MH  - Hybridomas
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Proteins/chemistry/genetics/metabolism
MH  - Virus Replication
EDAT- 2011/04/08 06:00
MHDA- 2011/10/27 06:00
CRDT- 2011/04/08 06:00
PHST- 2010/08/11 00:00 [received]
PHST- 2011/03/02 00:00 [revised]
PHST- 2011/03/06 00:00 [accepted]
PHST- 2011/04/08 06:00 [entrez]
PHST- 2011/04/08 06:00 [pubmed]
PHST- 2011/10/27 06:00 [medline]
AID - S0165-2427(11)00091-2 [pii]
AID - 10.1016/j.vetimm.2011.03.008 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2011 Jun 15;141(3-4):246-57. doi: 
      10.1016/j.vetimm.2011.03.008. Epub 2011 Mar 12.