PMID- 21471000
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20211020
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 22
IP  - 11
DP  - 2011 Jun 1
TI  - In neurons, activity-dependent association of dendritically transported mRNA 
      transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements.
PG  - 1864-77
LID - 10.1091/mbc.E10-11-0904 [doi]
AB  - In neurons certain mRNA transcripts are transported to synapses through 
      mechanisms that are not fully understood. Here we report that the heterogeneous 
      nuclear ribonucleoprotein CBF-A (CArG Box binding Factor A) facilitates dendritic 
      transport and localization of activity-regulated cytoskeleton-associated protein 
      (Arc), brain-derived neurotrophic factor (BDNF), and calmodulin-dependent protein 
      kinase II (CaMKIIalpha) mRNAs. We discovered that, in the adult mouse brain, CBF-A 
      has a broad distribution. In the nucleus, CBF-A was found at active transcription 
      sites and interchromosomal spaces and close to nuclear pores. In the cytoplasm, 
      CBF-A localized to dendrites as well as pre- and postsynaptic sites. CBF-A was 
      found in synaptosomal fractions, associated with Arc, BDNF, and CaMKIIalpha mRNAs. 
      Electrophoretic mobility shift assays demonstrated a direct interaction mediated 
      via their hnRNP A2 response element (A2RE)/RNA trafficking sequence (RTS) 
      elements located in the 3' untranslated regions. In situ hybridization and 
      microscopy on live hippocampal neurons showed that CBF-A is in dynamic granules 
      containing Arc, BDNF, and CaMKIIalpha mRNAs. N-methyl-D-aspartate (NMDA) and 
      alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) postsynaptic receptor 
      stimulation led to CBF-A accumulation in dendrites; increased Arc, BDNF, and 
      CaMKIIalpha mRNA levels; and increased amounts of transcripts coprecipitating with 
      CBF-A. Finally, CBF-A gene knockdown led to decreased mRNA levels. We propose 
      that CBF-A cotranscriptionally binds RTSs in Arc, BDNF, and CaMKIIalpha mRNAs and 
      follows the transcripts from genes to dendrites, promoting activity-dependent 
      nuclear sorting of transport-competent mRNAs.
FAU - Raju, Chandrasekhar S
AU  - Raju CS
AD  - Department of Cell and Molecular Biology, Karolinska Institutet, S-171 77 
      Stockholm, Sweden.
FAU - Fukuda, Nanaho
AU  - Fukuda N
FAU - Lopez-Iglesias, Carmen
AU  - Lopez-Iglesias C
FAU - Goritz, Christian
AU  - Goritz C
FAU - Visa, Neus
AU  - Visa N
FAU - Percipalle, Piergiorgio
AU  - Percipalle P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110406
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (CCAAT-Binding Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mtap2 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nfyb protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
SB  - IM
MH  - *3' Untranslated Regions
MH  - Animals
MH  - Axons/metabolism/ultrastructure
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - CCAAT-Binding Factor/genetics/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics
MH  - Cell Culture Techniques
MH  - Cell Nucleus/metabolism/ultrastructure
MH  - Cells, Cultured
MH  - Cytoplasmic Granules/metabolism
MH  - Cytoskeletal Proteins/genetics
MH  - Dendrites/*metabolism
MH  - Hippocampus/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microtubule-Associated Proteins/metabolism
MH  - Nerve Tissue Proteins/genetics
MH  - Neurons/*metabolism
MH  - Prosencephalon/cytology/metabolism
MH  - Protein Binding
MH  - RNA Interference
MH  - *RNA Transport
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Response Elements/*genetics
MH  - Synapses/metabolism/ultrastructure
PMC - PMC3103402
EDAT- 2011/04/08 06:00
MHDA- 2011/09/29 06:00
PMCR- 2011/08/16
CRDT- 2011/04/08 06:00
PHST- 2011/04/08 06:00 [entrez]
PHST- 2011/04/08 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
PHST- 2011/08/16 00:00 [pmc-release]
AID - mbc.E10-11-0904 [pii]
AID - E10-11-0904 [pii]
AID - 10.1091/mbc.E10-11-0904 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2011 Jun 1;22(11):1864-77. doi: 10.1091/mbc.E10-11-0904. Epub 2011 
      Apr 6.