PMID- 21472111
OWN - NLM
STAT- MEDLINE
DCOM- 20110725
LR  - 20220310
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 17
IP  - 11
DP  - 2011 Mar 21
TI  - Clinicopathologic significance of HER-2/neu protein expression and gene 
      amplification in gastric carcinoma.
PG  - 1501-6
LID - 10.3748/wjg.v17.i11.1501 [doi]
AB  - AIM: To study the HER-2/neu protein expression and gene amplification in gastric 
      carcinoma and their relation. METHODS: One hundred and forty-five formalin-fixed 
      and paraffin- embedded tumor tissue samples from Chinese gastric carcinoma 
      patients were studied with immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH) methods. Clinicopathologic data about all patients were 
      collected. RESULTS: The levels of HER-2 3+, HER-2 2+ and HER2 1+ were measurable 
      in 6.9%, 8.3% and 17.2% of the samples, respectively. No HER-2 was stained in 
      67.6% of the samples. FISH showed that HER-2 gene was amplified in 18 samples, 10 
      HER-2 3+ samples, 5 HER-2 2+ samples, and 3 HER-2 1+ samples with IHC staining. 
      HER-2 status was not correlated with the sex and age of patients, and tumor size, 
      location or differentiation, but with the depth of invasion, TNM stage, lymph 
      node and distant metastasis as well as histopathological classification of 
      gastric cancer (P < 0.05). CONCLUSION: All samples with IHC as HER-2 expression 
      should be analyzed with FISH. Detection of HER-2 gene amplification can assess 
      the malignant biological behaviors and prognosis of gastric cancer.
FAU - Yan, Shi-Yan
AU  - Yan SY
AD  - Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai 200092, China. shyyan2008phd@yahoo.com
FAU - Hu, Ying
AU  - Hu Y
FAU - Fan, Jian-Gao
AU  - Fan JG
FAU - Tao, Guo-Quan
AU  - Tao GQ
FAU - Lu, Yong-Ming
AU  - Lu YM
FAU - Cai, Xu
AU  - Cai X
FAU - Yu, Bao-Hua
AU  - Yu BH
FAU - Du, Yi-Qun
AU  - Du YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adenocarcinoma/chemistry/*genetics/pathology
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - China
MH  - Female
MH  - Fixatives
MH  - Formaldehyde
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Paraffin Embedding
MH  - Prognosis
MH  - Receptor, ErbB-2/analysis/*genetics
MH  - Stomach Neoplasms/chemistry/*genetics/pathology
MH  - Tissue Fixation/methods
PMC - PMC3070026
OTO - NOTNLM
OT  - Clinocopathologic significance
OT  - Fluorescence in situ hybridization
OT  - Gastric carcinoma
OT  - HER-2
OT  - Immunohistochemistry
EDAT- 2011/04/08 06:00
MHDA- 2011/07/26 06:00
PMCR- 2011/03/21
CRDT- 2011/04/08 06:00
PHST- 2010/08/08 00:00 [received]
PHST- 2010/12/13 00:00 [revised]
PHST- 2010/12/20 00:00 [accepted]
PHST- 2011/04/08 06:00 [entrez]
PHST- 2011/04/08 06:00 [pubmed]
PHST- 2011/07/26 06:00 [medline]
PHST- 2011/03/21 00:00 [pmc-release]
AID - 10.3748/wjg.v17.i11.1501 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2011 Mar 21;17(11):1501-6. doi: 10.3748/wjg.v17.i11.1501.