PMID- 21472544
OWN - NLM
STAT- MEDLINE
DCOM- 20110725
LR  - 20211020
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Linking)
VI  - 22
IP  - 1
DP  - 2011 Jan
TI  - Effects of electron-transfer coupled with collision-induced dissociation (ET/CID) 
      on doubly charged peptides and phosphopeptides.
PG  - 57-66
LID - 10.1007/s13361-010-0020-9 [doi]
AB  - Electron-transfer dissociation (ETD) is a useful peptide fragmentation technique 
      that can be applied to investigate post-translational modifications (PTMs), the 
      sequencing of highly hydrophilic peptides, and the identification of large 
      peptides and even intact proteins. In contrast to traditional fragmentation 
      methods, such as collision-induced dissociation (CID), ETD produces c- and 
      z(.)-type product ions by randomly cleaving the N-Calpha bonds. The disappointing 
      fragmentation efficiency of ETD for doubly charged peptides and phosphopeptide 
      ions has been improved by ETcaD (supplemental activation). However, the ETD data 
      derived from most database search algorithms yield low confidence scores due to 
      the presence of unreacted precursors and charge-reduced ions within MS/MS 
      spectra. In this work, we demonstrate that eight out of ten standard doubly 
      charged peptides and phosphopeptides can be effortlessly identified by 
      electron-transfer coupled with collision-induced dissociation (ET/CID) using the 
      SEQUEST algorithm without further spectral processing. ET/CID was performed with 
      the further dissociation of the charge-reduced ions isolated from ETD ion/ion 
      reactions. ET/CID had high fragmentation efficiency, which elevated the 
      confidence scores of doubly charged peptide and phosphospeptide sequencing. 
      ET/CID was found to be an effective fragmentation strategy in "bottom-up" 
      proteomic analysis.
CI  - (c) American Society for Mass Spectrometry, 2011
FAU - Liu, Chih-Wei
AU  - Liu CW
AD  - Institute of Molecular Biology, National Chung Hsing University, No. 250, 
      Kuo-Kuang Road, Taichung 402, Taiwan.
FAU - Lai, Chien-Chen
AU  - Lai CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110127
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphopeptides)
SB  - IM
MH  - Algorithms
MH  - Computational Biology
MH  - Mass Spectrometry/*methods
MH  - Peptide Fragments/*chemistry
MH  - Phosphopeptides/*chemistry
EDAT- 2011/04/08 06:00
MHDA- 2011/07/26 06:00
CRDT- 2011/04/08 06:00
PHST- 2010/07/26 00:00 [received]
PHST- 2010/10/14 00:00 [accepted]
PHST- 2010/10/13 00:00 [revised]
PHST- 2011/04/08 06:00 [entrez]
PHST- 2011/04/08 06:00 [pubmed]
PHST- 2011/07/26 06:00 [medline]
AID - 10.1007/s13361-010-0020-9 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2011 Jan;22(1):57-66. doi: 10.1007/s13361-010-0020-9. 
      Epub 2011 Jan 27.