PMID- 21476580
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20211020
IS  - 1520-4995 (Electronic)
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 50
IP  - 19
DP  - 2011 May 17
TI  - Combined genetic and metabolic manipulation of lipids in Rhodobacter sphaeroides 
      reveals non-phospholipid substitutions in fully active cytochrome c oxidase.
PG  - 3891-902
LID - 10.1021/bi1017039 [doi]
AB  - A specific requirement for lipids, particularly cardiolipin (CL), in cytochrome c 
      oxidase (CcO) has been reported in many previous studies using mainly in vitro 
      lipid removal approaches in mammalian systems. Our accompanying paper shows that 
      CcO produced in markedly CL-depleted Rhodobacter sphaeroides displays wild-type 
      properties in all respects, likely allowed by quantitative substitution with 
      other negatively charged lipids. To further examine the structural basis for the 
      lipid requirements of R. sphaeroides CcO and the extent of interchangeability 
      between lipids, we employed a metabolic approach to enhance the alteration of the 
      lipid profiles of the CcO-expressing strains of R. sphaeroides in vivo using a 
      phosphate-limiting growth medium in addition to the CL-deficient mutation. 
      Strikingly, the purified CcO produced under these conditions still maintained 
      wild-type function and characteristics, in spite of even greater depletion of 
      cardiolipin compared to that of the CL-deficient mutant alone (undetectable by 
      MS) and drastically altered profiles of all the phospholipids and 
      non-phospholipids. The lipids in the membrane and in the purified CcO were 
      identified and quantified by ESI and MALDI mass spectrometry and tandem mass 
      spectrometry. Comparison between the molecular structures of those lipids that 
      showed major changes provides new insight into the structural rationale for the 
      flexible lipid requirements of CcO from R. sphaeroides and reveals a more 
      comprehensive interchangeability network between different phospholipids and 
      non-phospholipids.
FAU - Zhang, Xi
AU  - Zhang X
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, Michigan 48824, United States.
FAU - Hiser, Carrie
AU  - Hiser C
FAU - Tamot, Banita
AU  - Tamot B
FAU - Benning, Christoph
AU  - Benning C
FAU - Reid, Gavin E
AU  - Reid GE
FAU - Ferguson-Miller, Shelagh M
AU  - Ferguson-Miller SM
LA  - eng
GR  - R01 GM026916/GM/NIGMS NIH HHS/United States
GR  - R37 GM026916/GM/NIGMS NIH HHS/United States
GR  - R37 GM026916-31/GM/NIGMS NIH HHS/United States
GR  - R01 GM26916/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110422
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Membrane Lipids)
RN  - 0 (Phosphates)
RN  - 0 (Phospholipids)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Electron Transport Complex IV/chemistry/*genetics/*metabolism
MH  - Enzyme Activation/genetics
MH  - Enzyme Stability/genetics
MH  - Membrane Lipids/chemistry/genetics/metabolism
MH  - Oxidation-Reduction
MH  - Phosphates/chemistry/metabolism
MH  - Phospholipids/chemistry/*genetics/*metabolism
MH  - Rhodobacter sphaeroides/*enzymology/genetics/metabolism
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spectrophotometry, Ultraviolet
MH  - Tandem Mass Spectrometry
PMC - PMC3097905
MID - NIHMS291507
EDAT- 2011/04/12 06:00
MHDA- 2011/08/02 06:00
PMCR- 2012/05/17
CRDT- 2011/04/12 06:00
PHST- 2011/04/12 06:00 [entrez]
PHST- 2011/04/12 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
PHST- 2012/05/17 00:00 [pmc-release]
AID - 10.1021/bi1017039 [doi]
PST - ppublish
SO  - Biochemistry. 2011 May 17;50(19):3891-902. doi: 10.1021/bi1017039. Epub 2011 Apr 
      22.