PMID- 21478008
OWN - NLM
STAT- MEDLINE
DCOM- 20160701
LR  - 20141013
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 9
DP  - 2011 May 15
TI  - Glucose biosensor based on covalent immobilization of enzyme in sol-gel composite 
      film combined with Prussian blue/carbon nanotubes hybrid.
PG  - 3973-6
LID - 10.1016/j.bios.2011.03.007 [doi]
AB  - A novel electrochemical glucose biosensor was developed based on in situ covalent 
      immobilization of glucose oxidase (GOx) by one-pot chitosan (CS)-incorporated 
      sol-gel process in the presence of Prussian blue deposited multi-walled carbon 
      nanotubes hybrids (PB/MWNTs) using 3-isocyanatopropyltriethoxysilane (ICPTES) as 
      both a sol-gel precursor and a covalent coupling agent for GOx and CS. The 
      electrode modified with the PB/MWNTs-GOx-CS-ICPTES sol-gel composite film showed 
      good electrical conductivity and effective low-potential electron transfer 
      mediation toward H2O2 reduction attributed to the incorporation of PB/MWNTs. The 
      biosensor exhibited a linear response to glucose in the concentration range from 
      2.5x10(-5) to 1.3x10(-3) M with a correlation coefficient of 0.9998, a detection 
      limit of 7.5x10(-6) M, a low response time (10s ), good sensitivity and high 
      anti-interference ability. Compared with the control biosensor based on the 
      traditional tetraethoxysilane derived sol-gel composite film, the biosensor 
      showed a similarly small apparent Michaelis-Menten constant of 3.67 mM but much 
      higher electrochemical and biosensing stability.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Fu, Guanglei
AU  - Fu G
AD  - Nanomedicine and Biosensor Laboratory, School of Sciences, State Key Laboratory 
      of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 
      150001, PR China.
FAU - Yue, Xiuli
AU  - Yue X
FAU - Dai, Zhifei
AU  - Dai Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110317
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Ferrocyanides)
RN  - 0 (Nanotubes, Carbon)
RN  - 9012-76-4 (Chitosan)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - IY9XDZ35W2 (Glucose)
RN  - TLE294X33A (ferric ferrocyanide)
SB  - IM
MH  - *Biosensing Techniques
MH  - Chitosan/chemistry
MH  - Electrochemistry
MH  - Enzymes, Immobilized/chemistry
MH  - Ferrocyanides/chemistry
MH  - Glucose/chemistry/*isolation & purification
MH  - Glucose Oxidase/chemistry
MH  - Nanotubes, Carbon/*chemistry
EDAT- 2011/04/12 06:00
MHDA- 2016/07/02 06:00
CRDT- 2011/04/12 06:00
PHST- 2010/12/04 00:00 [received]
PHST- 2011/03/05 00:00 [revised]
PHST- 2011/03/09 00:00 [accepted]
PHST- 2011/04/12 06:00 [entrez]
PHST- 2011/04/12 06:00 [pubmed]
PHST- 2016/07/02 06:00 [medline]
AID - S0956-5663(11)00145-X [pii]
AID - 10.1016/j.bios.2011.03.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2011 May 15;26(9):3973-6. doi: 10.1016/j.bios.2011.03.007. 
      Epub 2011 Mar 17.