PMID- 21478444
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20220321
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 23
IP  - 4
DP  - 2011 Apr
TI  - The interconversion of UDP-arabinopyranose and UDP-arabinofuranose is 
      indispensable for plant development in Arabidopsis.
PG  - 1373-90
LID - 10.1105/tpc.111.083931 [doi]
AB  - L-Ara, an important constituent of plant cell walls, is found predominantly in 
      the furanose rather than in the thermodynamically more stable pyranose form. 
      Nucleotide sugar mutases have been demonstrated to interconvert 
      UDP-Larabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in rice 
      (Oryza sativa). These enzymes belong to a small gene family encoding the 
      previously named Reversibly Glycosylated Proteins (RGPs). RGPs are plant-specific 
      cytosolic proteins that tend to associate with the endomembrane system. In 
      Arabidopsis thaliana, the RGP protein family consists of five closely related 
      members. We characterized all five RGPs regarding their expression pattern and 
      subcellular localizations in transgenic Arabidopsis plants. Enzymatic activity 
      assays of recombinant proteins expressed in Escherichia coli identified three of 
      the Arabidopsis RGP protein family members as UDP-L-Ara mutases that catalyze the 
      formation of UDP-Araf from UDP-Arap. Coimmunoprecipitation and subsequent liquid 
      chromatography-electrospray ionization-tandem mass spectrometry analysis revealed 
      a distinct interaction network between RGPs in different Arabidopsis organs. 
      Examination of cell wall polysaccharide preparations from RGP1 and RGP2 knockout 
      mutants showed a significant reduction in total L-Ara content (12-31%) compared 
      with wild-type plants. Concomitant downregulation of RGP1 and RGP2 expression 
      results in plants almost completely deficient in cell wall-derived L-Ara and 
      exhibiting severe developmental defects.
FAU - Rautengarten, Carsten
AU  - Rautengarten C
AD  - Joint BioEnergy Institute, Feedstocks Division, Lawrence Berkeley National 
      Laboratory, Emeryville, California 94608, USA.
FAU - Ebert, Berit
AU  - Ebert B
FAU - Herter, Thomas
AU  - Herter T
FAU - Petzold, Christopher J
AU  - Petzold CJ
FAU - Ishii, Tadashi
AU  - Ishii T
FAU - Mukhopadhyay, Aindrila
AU  - Mukhopadhyay A
FAU - Usadel, Bjorn
AU  - Usadel B
FAU - Scheller, Henrik Vibe
AU  - Scheller HV
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110408
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Carbohydrates)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Uridine Diphosphate Sugars)
RN  - 14697-41-7 (uridine diphosphate arabinose)
RN  - EC 5.4.- (Intramolecular Transferases)
SB  - IM
MH  - Arabidopsis/enzymology/genetics/*growth & development/*metabolism
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Carbohydrate Metabolism
MH  - Carbohydrates/analysis
MH  - Cell Wall/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Plant
MH  - Intramolecular Transferases/metabolism
MH  - Multiprotein Complexes/metabolism
MH  - Plants, Genetically Modified
MH  - Protein Transport
MH  - Recombinant Fusion Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Subcellular Fractions/metabolism
MH  - Uridine Diphosphate Sugars/*metabolism
PMC - PMC3101560
EDAT- 2011/04/12 06:00
MHDA- 2011/09/20 06:00
PMCR- 2011/04/08
CRDT- 2011/04/12 06:00
PHST- 2011/04/12 06:00 [entrez]
PHST- 2011/04/12 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
PHST- 2011/04/08 00:00 [pmc-release]
AID - tpc.111.083931 [pii]
AID - 083931 [pii]
AID - 10.1105/tpc.111.083931 [doi]
PST - ppublish
SO  - Plant Cell. 2011 Apr;23(4):1373-90. doi: 10.1105/tpc.111.083931. Epub 2011 Apr 8.