PMID- 21479786
OWN - NLM
STAT- MEDLINE
DCOM- 20111021
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 354
IP  - 1-2
DP  - 2011 Aug
TI  - Identification and functional characterization of a putative 17beta-hydroxysteroid 
      dehydrogenase 12 in abalone (Haliotis diversicolor supertexta).
PG  - 123-33
LID - 10.1007/s11010-011-0811-8 [doi]
AB  - The 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are key enzymes in the 
      downstream process of steroid hormone biosynthesis. To date, relatively little is 
      known about the role of 17beta-HSDs in marine gastropods. In the present study, a 
      putative cDNA sequence encoding type 12 17beta-HSD (17beta-HSD-12) was identified in 
      abalone (Haliotis diversicolor supertexta). The full-length cDNA was 1,978 bp, 
      including an open reading frame (ORF) of 963 bp that encoded a protein of 321 
      amino acids. Comparative structural analysis revealed that abalone 17beta-HSD-12 
      shared 39.8-42.8% amino acid identity with other 17beta-HSD-12 homologues and that 
      the functional domains were well conserved. Phylogenetic analysis revealed that 
      abalone 17beta-HSD-12 belonged to the short-chain dehydrogenases/reductases (SDRs) 
      family. Functional analysis following transient transfection of the ORF in human 
      embryonic kidney-293 (HEK-293) cells indicated that abalone 17beta-HSD-12 had the 
      ability to convert estrone (E1) into estradiol (E2). Expression analysis in vivo 
      demonstrated that abalone 17beta-HSD-12 was differentially expressed during the 
      three reproductive stages (pre-spawning, spawning, and post-spawning). These 
      results indicate that abalone 17beta-HSD-12 is an SDR family member with a key role 
      in steroidogenesis during the reproductive period.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Life Sciences Division, Graduate School at Shenzhen, Campus of Tsinghua 
      University, Shenzhen, Room 304, Building L, University Town, Guangdong Province, 
      People's Republic of China.
FAU - Gao, Yun-Feng
AU  - Gao YF
FAU - Li, Lei
AU  - Li L
FAU - Zhai, Hong-Ning
AU  - Zhai HN
FAU - Tan, Shang-Jin
AU  - Tan SJ
FAU - Cai, Zhong-Hua
AU  - Cai ZH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110409
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Enzyme Assays
MH  - Gastrointestinal Tract/metabolism
MH  - Gastropoda/*enzymology
MH  - Gonads/metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Phylogeny
MH  - Protein Structure, Tertiary
MH  - Sequence Alignment
MH  - Transcription, Genetic
EDAT- 2011/04/12 06:00
MHDA- 2011/10/22 06:00
CRDT- 2011/04/12 06:00
PHST- 2011/01/11 00:00 [received]
PHST- 2011/03/24 00:00 [accepted]
PHST- 2011/04/12 06:00 [entrez]
PHST- 2011/04/12 06:00 [pubmed]
PHST- 2011/10/22 06:00 [medline]
AID - 10.1007/s11010-011-0811-8 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2011 Aug;354(1-2):123-33. doi: 10.1007/s11010-011-0811-8. Epub 
      2011 Apr 9.