PMID- 21482698
OWN - NLM
STAT- MEDLINE
DCOM- 20110718
LR  - 20211020
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 208
IP  - 5
DP  - 2011 May 9
TI  - Dynamic cortical actin remodeling by ERM proteins controls BCR microcluster 
      organization and integrity.
PG  - 1055-68
LID - 10.1084/jem.20101125 [doi]
AB  - Signaling microclusters are a common feature of lymphocyte activation. However, 
      the mechanisms controlling the size and organization of these discrete structures 
      are poorly understood. The Ezrin-Radixin-Moesin (ERM) proteins, which link plasma 
      membrane proteins with the actin cytoskeleton and regulate the steady-state 
      diffusion dynamics of the B cell receptor (BCR), are transiently dephosphorylated 
      upon antigen receptor stimulation. In this study, we show that the ERM proteins 
      ezrin and moesin influence the organization and integrity of BCR microclusters. 
      BCR-driven inactivation of ERM proteins is accompanied by a temporary increase in 
      BCR diffusion, followed by BCR immobilization. Disruption of ERM protein function 
      using dominant-negative or constitutively active ezrin constructs or knockdown of 
      ezrin and moesin expression quantitatively and qualitatively alters BCR 
      microcluster formation, antigen aggregation, and downstream BCR signal 
      transduction. Chemical inhibition of actin polymerization also altered the 
      structure and integrity of BCR microclusters. Together, these findings highlight 
      a crucial role for the cortical actin cytoskeleton during B cell spreading and 
      microcluster formation and function.
FAU - Treanor, Bebhinn
AU  - Treanor B
AD  - Lymphocyte Interaction Laboratory, London Research Institute, Cancer Research UK, 
      London WC2A 3PX, England, UK. bebhinn.treanor@cancer.org.uk
FAU - Depoil, David
AU  - Depoil D
FAU - Bruckbauer, Andreas
AU  - Bruckbauer A
FAU - Batista, Facundo D
AU  - Batista FD
LA  - eng
GR  - Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110411
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Actins)
RN  - 0 (Antigens)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (ezrin)
RN  - 144131-77-1 (moesin)
SB  - IM
MH  - Actins/genetics/*immunology/metabolism
MH  - Animals
MH  - Antigens/genetics/immunology
MH  - B-Lymphocytes/*immunology/metabolism
MH  - Cytoskeletal Proteins/genetics/*immunology/metabolism
MH  - Cytoskeleton/genetics/immunology/metabolism
MH  - Gene Expression Regulation/genetics/immunology
MH  - Gene Knockdown Techniques
MH  - Lymphocyte Activation/genetics/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Microfilament Proteins/genetics/*immunology/metabolism
MH  - Phosphorylation/genetics/immunology
MH  - Receptors, Antigen, B-Cell/genetics/*immunology/metabolism
MH  - Signal Transduction/genetics/*immunology
PMC - PMC3092358
EDAT- 2011/04/13 06:00
MHDA- 2011/07/19 06:00
PMCR- 2011/11/09
CRDT- 2011/04/13 06:00
PHST- 2011/04/13 06:00 [entrez]
PHST- 2011/04/13 06:00 [pubmed]
PHST- 2011/07/19 06:00 [medline]
PHST- 2011/11/09 00:00 [pmc-release]
AID - jem.20101125 [pii]
AID - 20101125 [pii]
AID - 10.1084/jem.20101125 [doi]
PST - ppublish
SO  - J Exp Med. 2011 May 9;208(5):1055-68. doi: 10.1084/jem.20101125. Epub 2011 Apr 
      11.