PMID- 21485540
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20131121
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 17
IP  - 3
DP  - 2011 Mar
TI  - [Establishment of a rat model of flutamide-induced cryptorchidism with remaining 
      gonocytes].
PG  - 208-13
AB  - OBJECTIVE: To observe the effects of flutamide (Flu) on the development of 
      testicular germocytes in SD rats, and to establish a rat model for further 
      researches on the maldevelopment of cryptorchidism gonocytes (Go). METHODS: 
      Pregnant SD rats were subcutaneously injected with Flu from gestational day (GD) 
      12 to 21 to establish a model of cryptorchidism. The testes of the newborns were 
      harvested on postnatal day (PD) 1, 10, 20 and 80 for observation of their 
      morphological and histological changes by HE staining and detection of the 
      expression of neural cell adhesion molecules (NCAM) by immunohistochemistry and 
      RT-PCR. RESULTS: Flu induced 43.9% (29/66) of cryptorchidism in the exposed rats. 
      Significant differences were found in the testicular weight and organ coefficient 
      between the Flu and the control groups on PD 20 and 80. Gos remained in the 
      center of seminiferous tubules of the Flu-induced testis on PD 10, and in the 
      center of seminiferous tubules in the cryptorchids' testicular tissues on PD 20 
      and 80. Immunohistochemistry showed the expression of NCAM on the membrane of the 
      remaining Gos, and RT-PCR revealed significantly up-regulated expression of NCAM 
      mRNA in the Flu-induced testes on PD 10 and 20 as compared with the controls (P < 
      0.05). CONCLUSION: A rat model of Flu-induced cryptorchidism with remaining Gos 
      was successfully established, which could be used to study the mechanism and 
      management of the maldevelopment of cryptorchidism gonocytes.
FAU - Ma, Chao
AU  - Ma C
AD  - Key Laboratory for Children's Development and Disorders of the Chinese Ministry 
      of Education/Department of Urology, Children's Hospital of Chongqing Medical 
      University, Chongqing 400014, China.
FAU - He, Da-wei
AU  - He DW
FAU - Zhao, Dan
AU  - Zhao D
FAU - He, Wen-fei
AU  - He WF
FAU - Zhang, Yong-bo
AU  - Zhang YB
FAU - Wei, Guang-hui
AU  - Wei GH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 76W6J0943E (Flutamide)
SB  - IM
MH  - Animals
MH  - Cryptorchidism/chemically induced/*pathology
MH  - Female
MH  - Flutamide/*adverse effects
MH  - Male
MH  - Neural Cell Adhesion Molecules/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testis/drug effects/metabolism/*pathology
EDAT- 2011/04/14 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/04/14 06:00
PHST- 2011/04/14 06:00 [entrez]
PHST- 2011/04/14 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2011 Mar;17(3):208-13.