PMID- 21487199
OWN - NLM
STAT- MEDLINE
DCOM- 20110722
LR  - 20190608
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Linking)
VI  - 26
IP  - 1
DP  - 2011
TI  - Enrichment of a novel marine ammonia-oxidizing archaeon obtained from sand of an 
      eelgrass zone.
PG  - 23-9
AB  - Ammonia-oxidizing archaea (AOA) are generally cultivated at ammonium 
      concentrations of less than 2 mM. The physiology and abundance in the environment 
      of AOA suggest an important role in the nitrogen cycle. We report here a novel 
      marine ammonia-oxidizing crenarchaeote, strain NM25 belonged to 'Candidatus 
      Nitrosopumilus', that was enriched from coastal sand of an eelgrass zone and grew 
      in a medium containing 15 mM ammonium at 30 degrees C. A phylogenetic analysis based on 
      the 16S rRNA gene revealed this crenarchaeote was related to the 
      ammonia-oxidizing archaeon 'Candidatus Nitrosopumilus maritimus' strain SCM1, 
      with 98.5% identity. The ammonia monooxygenase subunit A (amoA) gene of strain 
      NM25 was less closely related to that of known cultivable AOA (>95%) and 
      environmental clones (>97%). This finding suggests the existence of AOA adapted 
      to high ammonium-containing environments.
FAU - Matsutani, Naoki
AU  - Matsutani N
AD  - Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, 
      Fujisawa, Kanagawa 252-0880, Japan.
FAU - Nakagawa, Tatsunori
AU  - Nakagawa T
FAU - Nakamura, Kyoko
AU  - Nakamura K
FAU - Takahashi, Reiji
AU  - Takahashi R
FAU - Yoshihara, Kiyoshi
AU  - Yoshihara K
FAU - Tokuyama, Tatsuaki
AU  - Tokuyama T
LA  - eng
SI  - GENBANK/AB546961
SI  - GENBANK/AB546962
SI  - GENBANK/AB546963
SI  - GENBANK/AB546964
SI  - GENBANK/AB546965
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (Archaeal Proteins)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/classification/genetics/*isolation & purification/*metabolism
MH  - Archaeal Proteins/genetics/metabolism
MH  - Geologic Sediments/*microbiology
MH  - Hydrocharitaceae/*microbiology
MH  - Molecular Sequence Data
MH  - Oxidoreductases/genetics/metabolism
MH  - Phylogeny
MH  - Seawater/*microbiology
MH  - Silicon Dioxide/analysis
EDAT- 2011/04/14 06:00
MHDA- 2011/07/23 06:00
CRDT- 2011/04/14 06:00
PHST- 2011/04/14 06:00 [entrez]
PHST- 2011/04/14 06:00 [pubmed]
PHST- 2011/07/23 06:00 [medline]
AID - JST.JSTAGE/jsme2/ME10156 [pii]
AID - 10.1264/jsme2.me10156 [doi]
PST - ppublish
SO  - Microbes Environ. 2011;26(1):23-9. doi: 10.1264/jsme2.me10156.