PMID- 21488875
OWN - NLM
STAT- MEDLINE
DCOM- 20111102
LR  - 20110624
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 46
IP  - 4
DP  - 2011 Aug
TI  - The possible mechanism of preterm birth associated with periodontopathic 
      Porphyromonas gingivalis.
PG  - 497-504
LID - 10.1111/j.1600-0765.2011.01366.x [doi]
AB  - BACKGROUND AND OBJECTIVE: Previous studies have shown that Porphyromonas 
      gingivalis is found in the amniotic fluid and placentae of pregnant women with 
      some obstetric diseases. However, the biological effects of P. gingivalis on 
      intrauterine tissues remain unclear. The aim of this study was to investigate the 
      presence of P. gingivalis in chorionic tissues from hospitalized high-risk 
      pregnant women, and the effects of P. gingivalis lipopolysaccharide on the 
      production of proinflammatory molecules in human chorion-derived cells. MATERIAL 
      AND METHODS: Twenty-three subjects were selected from Japanese hospitalized 
      high-risk pregnant women. The presence of P. gingivalis in chorionic tissues was 
      analyzed by PCR. Cultured chorion-derived cells or Toll-like receptor-2 (TLR-2) 
      gene-silenced chorion-derived cells were stimulated with P. gingivalis 
      lipopolysaccharide. Real-time PCR was performed to evaluate TLR-2 and Toll-like 
      receptor-4 (TLR-4) mRNA expression in the cells. Levels of interleukin-6 and 
      interleukin-8 in culture supernatants of the chorion-derived cells were measured 
      by ELISA. RESULTS: P. gingivalis DNA was detected in chorionic tissues from two 
      women with threatened preterm labor, two with multiple pregnancy and two with 
      placenta previa. Stimulation of chorion-derived cells with P. gingivalis 
      lipopolysaccharide significantly increased TLR-2 mRNA expression, whereas TLR-4 
      mRNA expression was not changed. P. gingivalis lipopolysaccharide induced 
      interleukin-6 and interleukin-8 production in chorion-derived cells, but the P. 
      gingivalis lipopolysaccharide-induced interleukin-6 and interleukin-8 production 
      was reduced in TLR-2 gene-silenced chorion-derived cells. CONCLUSION: Our results 
      suggest that P. gingivalis can be detected in chorionic tissues of hospitalized 
      high-risk pregnant women, and that P. gingivalis lipopolysaccharide induces 
      interleukin-6 and interleukin-8 production via TLR-2 in chorion-derived cells.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Hasegawa-Nakamura, K
AU  - Hasegawa-Nakamura K
AD  - Division of Periodontology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan. hasegawa@dent.kagoshima-u.ac.jp
FAU - Tateishi, F
AU  - Tateishi F
FAU - Nakamura, T
AU  - Nakamura T
FAU - Nakajima, Y
AU  - Nakajima Y
FAU - Kawamata, K
AU  - Kawamata K
FAU - Douchi, T
AU  - Douchi T
FAU - Hatae, M
AU  - Hatae M
FAU - Noguchi, K
AU  - Noguchi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110413
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Chorion/drug effects/*microbiology
MH  - Dental Plaque/microbiology
MH  - Escherichia coli
MH  - Female
MH  - Gene Silencing
MH  - Gingivitis/classification
MH  - Hospitalization
MH  - Humans
MH  - Inflammation Mediators/analysis
MH  - Interleukin-6/analysis
MH  - Interleukin-8/analysis
MH  - Lipopolysaccharides/pharmacology
MH  - Periodontal Attachment Loss/classification
MH  - Periodontal Diseases/*microbiology
MH  - Periodontal Pocket/classification
MH  - Periodontitis/classification
MH  - Placenta Previa/microbiology
MH  - Porphyromonas gingivalis/*isolation & purification
MH  - Pregnancy
MH  - Pregnancy, High-Risk
MH  - Pregnancy, Multiple
MH  - Premature Birth/*microbiology
MH  - Saliva/microbiology
MH  - Toll-Like Receptor 2/analysis/genetics
MH  - Toll-Like Receptor 4/analysis
MH  - Young Adult
EDAT- 2011/04/15 06:00
MHDA- 2011/11/04 06:00
CRDT- 2011/04/15 06:00
PHST- 2011/04/15 06:00 [entrez]
PHST- 2011/04/15 06:00 [pubmed]
PHST- 2011/11/04 06:00 [medline]
AID - 10.1111/j.1600-0765.2011.01366.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2011 Aug;46(4):497-504. doi: 10.1111/j.1600-0765.2011.01366.x. 
      Epub 2011 Apr 13.