PMID- 21489581
OWN - NLM
STAT- MEDLINE
DCOM- 20111121
LR  - 20191210
IS  - 1532-8600 (Electronic)
IS  - 0026-0495 (Linking)
VI  - 60
IP  - 10
DP  - 2011 Oct
TI  - Effect of carbohydrate-protein supplementation postexercise on rat muscle 
      glycogen synthesis and phosphorylation of proteins controlling glucose storage.
PG  - 1406-15
LID - 10.1016/j.metabol.2011.02.007 [doi]
AB  - To examine whether addition of protein to a carbohydrate supplement enhances 
      muscle glycogen synthesis, we compared the muscle glycogen concentrations of rats 
      that had been depleted of their muscle glycogen stores with a 3-hour swim and 
      immediately supplemented with a placebo (Con), carbohydrate (CHO), or 
      carbohydrate plus protein supplement (C+P). Rats were given either 0.9 g 
      carbohydrate per kilogram body mass for the CHO group or 0.9 g carbohydrate + 0.3 
      g protein per kilogram body mass for the C+P groups. Muscle samples of the red 
      and white quadriceps were excised immediately, 30 minutes, or 90 minutes 
      postexercise. Glycogen concentration of the C+P group was greater than that of 
      the CHO group at 90 minutes postexercise in both red (C+P, 28.3 +/- 2.6 micromol/g vs 
      CHO, 22.4 +/- 2.0 micromol/g; P < .05) and white (C+P, 24.9 +/- 2.4 micromol/g vs CHO, 17.64 
      +/- 1.5 micromol/g; P < .01) quadriceps. Protein kinase B phosphorylation was greater 
      in the C+P-30 group (the number following treatment group abbreviation refers to 
      time [in minutes] of euthanasia following exercise) than the sedentary control 
      and exercised control groups in red quadriceps at 30 minutes and in white 
      quadriceps at 90 minutes postexercise. This difference was not observed in the 
      CHO group. Phosphorylation of glycogen synthase was significantly reduced 30 
      minutes postexercise and returned to baseline levels by 90 minutes postexercise 
      in both CHO- and C+P-supplemented groups, with no difference between supplements. 
      These results demonstrated that the addition of protein to a carbohydrate 
      supplement will enhance the rate of muscle glycogen restoration postexercise and 
      may involve facilitation of the glucose transport process.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Hara, Daisuke
AU  - Hara D
AD  - Exercise Physiology and Metabolism Laboratory, Department of Kinesiology and 
      Health Education, University of Texas, Austin, TX 78712, USA.
FAU - Morrison, Paul J
AU  - Morrison PJ
FAU - Ding, Zhenping
AU  - Ding Z
FAU - Ivy, John L
AU  - Ivy JL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110412
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (Insulin)
RN  - 0 (Muscle Proteins)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Dietary Carbohydrates/*administration & dosage/pharmacology
MH  - Dietary Proteins/*administration & dosage/pharmacology
MH  - Dietary Supplements
MH  - Drug Administration Schedule
MH  - Glucose/*metabolism
MH  - Glycogen/*biosynthesis/metabolism
MH  - Insulin/blood
MH  - Male
MH  - Muscle Proteins/*metabolism/physiology
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - Phosphorylation/drug effects
MH  - *Physical Conditioning, Animal/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 2011/04/15 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/04/15 06:00
PHST- 2010/02/24 00:00 [received]
PHST- 2011/02/08 00:00 [revised]
PHST- 2011/02/10 00:00 [accepted]
PHST- 2011/04/15 06:00 [entrez]
PHST- 2011/04/15 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0026-0495(11)00045-X [pii]
AID - 10.1016/j.metabol.2011.02.007 [doi]
PST - ppublish
SO  - Metabolism. 2011 Oct;60(10):1406-15. doi: 10.1016/j.metabol.2011.02.007. Epub 
      2011 Apr 12.