PMID- 21489634
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20181201
IS  - 1873-6750 (Electronic)
IS  - 0160-4120 (Linking)
VI  - 37
IP  - 5
DP  - 2011 Jul
TI  - Enhancement of AhR-mediated activity of selected pollutants and their mixtures 
      after interaction with dissolved organic matter.
PG  - 960-4
LID - 10.1016/j.envint.2011.03.016 [doi]
AB  - Dissolved organic matter (DOM) in freshwaters is present at concentrations 
      ranging from 0.5 to 50 mg L(-)(1), and consists of various organic compounds, 
      including humic substances (HS). HS exert a variety of direct and indirect 
      biological effects, including interaction with the aryl hydrocarbon receptor 
      (AhR). AhR is a cytosolic receptor that binds various hydrophobic organic 
      compounds (HOCs) and mediates some of their toxic effects. In vitro effects of 
      binary mixtures of various DOM (mainly HS) with various HOCs on AhR-mediated 
      responses were studied by use of H4IIE-luc cells. Six out of 12 DOM activated the 
      AhR even at environmentally relevant concentrations (17 mg L(-)(1)). In simultaneous 
      exposures of H4IIE-luc cells to DOM (17 mg L(-)(1)) and each of the model compounds, 
      2,3,7,8-TCDD, PCB126, PCB169, benzo[a]pyrene, benzo[a]anthracene, 
      dibenz[a,h]anthracene, fluoranthene, a mixture of persistent organic pollutants 
      (POPs), a mixture of polycyclic aromatic hydrocarbons (PAHs), and a mixture of 
      all HOCs, either significant additive or facilitative effects were observed when 
      compared to activities of single HOCs. No significant decrease of effects due to 
      possible sorption of HOCs to DOM was observed, even in subsequent experiments 
      when HOCs+DOM mixtures were preincubated for six days before exposure to 
      H4IIE-luc. Thus, DOM does not seem to protect organisms against AhR-mediated 
      toxic effects of HOCs (as usually predicted due to sorption of HOCs on DOM), but 
      it can actually enhance their potency for AhR-mediated effects in some 
      situations.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Bittner, Michal
AU  - Bittner M
AD  - Research Centre for Toxic Compounds in the Environment, Masaryk University, 
      Kamenice 126/3, CZ62500 Brno, Czech Republic. bittner@recetox.muni.cz
FAU - Macikova, Petra
AU  - Macikova P
FAU - Giesy, John P
AU  - Giesy JP
FAU - Hilscherova, Klara
AU  - Hilscherova K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110413
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
RN  - 0 (Anthracenes)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorenes)
RN  - 0 (Humic Substances)
RN  - 0 (Organic Chemicals)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 360UOL779Z (fluoranthene)
RN  - EH46A1TLD7 (anthracene)
SB  - IM
MH  - Animals
MH  - Anthracenes/chemistry/metabolism/toxicity
MH  - Benzo(a)pyrene/chemistry/metabolism/toxicity
MH  - Cell Line, Tumor
MH  - Environmental Pollutants/chemistry/metabolism/*toxicity
MH  - Fluorenes/chemistry/metabolism/toxicity
MH  - Fresh Water/chemistry
MH  - Humic Substances/analysis
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Organic Chemicals/chemistry/*metabolism/toxicity
MH  - Polychlorinated Dibenzodioxins/chemistry/metabolism/toxicity
MH  - Polycyclic Aromatic Hydrocarbons/chemistry/metabolism/toxicity
MH  - Rats
MH  - Receptors, Aryl Hydrocarbon/*metabolism
EDAT- 2011/04/15 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/04/15 06:00
PHST- 2010/07/13 00:00 [received]
PHST- 2011/02/09 00:00 [revised]
PHST- 2011/03/17 00:00 [accepted]
PHST- 2011/04/15 06:00 [entrez]
PHST- 2011/04/15 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - S0160-4120(11)00087-0 [pii]
AID - 10.1016/j.envint.2011.03.016 [doi]
PST - ppublish
SO  - Environ Int. 2011 Jul;37(5):960-4. doi: 10.1016/j.envint.2011.03.016. Epub 2011 
      Apr 13.