PMID- 21494579
OWN - NLM
STAT- MEDLINE
DCOM- 20110613
LR  - 20220408
IS  - 2210-7762 (Print)
VI  - 204
IP  - 2
DP  - 2011 Feb
TI  - Enhanced detection of chromosomal abnormalities in chronic lymphocytic leukemia 
      by conventional cytogenetics using CpG oligonucleotide in combination with 
      pokeweed mitogen and phorbol myristate acetate.
PG  - 77-83
LID - 10.1016/j.cancergen.2010.12.006 [doi]
AB  - Reproducible cytogenetic analysis in CLL has been limited by the inability to 
      obtain reliable metaphase cells for analysis. CpG oligonucleotide and cytokine 
      stimulation have been shown to improve metaphase analysis of CLL cytogenetic 
      abnormalities, but is limited by variability in the cytokine receptor levels, 
      stability and biological activity of the cytokine in culture conditions and high 
      costs associated with these reagents. We report here use of a novel, stable CpG, 
      GNKG168 along with pokeweed mitogen (PWM) and phorbol 12-myristate 13-acetate 
      (PMA) for conventional cytogenetic assessment in CLL. We demonstrate that the 
      combined use of GNKG168+PWM/PMA increased the sensitivity of detection of 
      chromosomal abnormalities compared to PWM/PMA (n=207, odds ratio=2.2, p=0.0002) 
      and GNKG168 (n=219, odds ratio=1.5, p=0.0452). Further, a significant increase in 
      sensitivity to detect complexity >/=3 with GNKG168+PWM/PMA compared to GNKG168 
      alone (odds ratio 8.0, p=0.0022) or PWM/PMA alone (odds ratio 9.6, p=0.0007) was 
      observed. The trend toward detection of higher complexity was significantly 
      greater with GNKG168+PWM/PMA compared to GNKG168 alone (p=0.0412). The increased 
      sensitivity was mainly attributed to the addition of PWM/PMA with GNKG168 because 
      GNKG168 alone showed no difference in sensitivity for detection of complex 
      abnormalities (p=0.17). Comparison of fluorescence in situ hybridization (FISH) 
      results with karyotypic results showed a high degree of consistency, although 
      some complex karyotypes were present in cases with no adverse FISH abnormality. 
      These studies provide evidence for potential use of GNKG168 in combination with 
      PWM and PMA in karyotypic analysis of CLL patient samples to better identify 
      chromosomal abnormalities for risk stratification.
FAU - Muthusamy, Natarajan
AU  - Muthusamy N
AD  - Division of Hematology, The OSU Comprehensive Cancer Center, The Ohio State 
      University, Columbus, USA. raj.muthusamy@osumc.edu
FAU - Breidenbach, Heather
AU  - Breidenbach H
FAU - Andritsos, Leslie
AU  - Andritsos L
FAU - Flynn, Joseph
AU  - Flynn J
FAU - Jones, Jeffrey
AU  - Jones J
FAU - Ramanunni, Asha
AU  - Ramanunni A
FAU - Mo, Xiaokui
AU  - Mo X
FAU - Jarjoura, David
AU  - Jarjoura D
FAU - Byrd, John C
AU  - Byrd JC
FAU - Heerema, Nyla A
AU  - Heerema NA
LA  - eng
GR  - P01 CA95426/CA/NCI NIH HHS/United States
GR  - P50 CA140158/CA/NCI NIH HHS/United States
GR  - 1K12 CA133250/CA/NCI NIH HHS/United States
GR  - P01 CA101956/CA/NCI NIH HHS/United States
GR  - P50-CA140158/CA/NCI NIH HHS/United States
GR  - P50 CA140158-03/CA/NCI NIH HHS/United States
GR  - P01 CA095426/CA/NCI NIH HHS/United States
GR  - K12 CA133250/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
RN  - 0 (GNKG168)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Pokeweed Mitogens)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Humans
MH  - Karyotyping/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/*genetics
MH  - Middle Aged
MH  - Oligodeoxyribonucleotides
MH  - Pokeweed Mitogens
MH  - Sensitivity and Specificity
MH  - Tetradecanoylphorbol Acetate
PMC - PMC3073597
MID - NIHMS261046
EDAT- 2011/04/16 06:00
MHDA- 2011/06/15 06:00
PMCR- 2012/02/01
CRDT- 2011/04/16 06:00
PHST- 2011/04/16 06:00 [entrez]
PHST- 2011/04/16 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
PHST- 2012/02/01 00:00 [pmc-release]
AID - S2210-7762(10)00604-6 [pii]
AID - 10.1016/j.cancergen.2010.12.006 [doi]
PST - ppublish
SO  - Cancer Genet. 2011 Feb;204(2):77-83. doi: 10.1016/j.cancergen.2010.12.006.