PMID- 21496223
OWN - NLM
STAT- MEDLINE
DCOM- 20110831
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 8
DP  - 2011 Apr 15
TI  - A 5'-proximal stem-loop structure of 5' untranslated region of porcine 
      reproductive and respiratory syndrome virus genome is key for virus replication.
PG  - 172
LID - 10.1186/1743-422X-8-172 [doi]
AB  - BACKGROUND: It has been well documented that the 5' untranslated region (5' UTR) 
      of many positive-stranded RNA viruses contain key cis-acting regulatory 
      sequences, as well as high-order structural elements. Little is known for such 
      regulatory elements controlling porcine arterivirus replication. We investigated 
      the roles of a conserved stem-loop 2 (SL2) that resides in the 5'UTR of the 
      genome of a type II porcine reproductive and respiratory syndrome virus (PRRSV). 
      RESULTS: We provided genetic evidences demonstrating that 1) the SL2 in type II 
      PRRSV 5' UTR, N-SL2, could be structurally and functionally substituted by its 
      counterpart in type I PRRSV, E-SL2; 2) the functionality of N-SL2 was dependent 
      upon the G-C rich stem structure, while the ternary-loop size was irrelevant to 
      RNA synthesis; 3) serial deletions showed that the stem integrity of N-SL2 was 
      crucial for subgenomic mRNA synthesis; and 4) when extensive base-pairs in the 
      stem region was deleted, an alternative N-SL2-like structure with different 
      sequence was utilized for virus replication. CONCLUSION: Taken together, we 
      concluded that the phylogenetically conserved SL2 in the 5' UTR was crucial for 
      PRRSV virus replication, subgenomic mRNA synthesis in particular.
FAU - Lu, Jiaqi
AU  - Lu J
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
FAU - Gao, Fei
AU  - Gao F
FAU - Wei, Zuzhang
AU  - Wei Z
FAU - Liu, Ping
AU  - Liu P
FAU - Liu, Changlong
AU  - Liu C
FAU - Zheng, Haihong
AU  - Zheng H
FAU - Li, Yanhua
AU  - Li Y
FAU - Lin, Tao
AU  - Lin T
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110415
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Conserved Sequence
MH  - Cricetinae
MH  - *Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*physiology
MH  - RNA, Viral/*genetics
MH  - Recombination, Genetic
MH  - Sequence Deletion
MH  - *Virus Replication
PMC - PMC3096946
EDAT- 2011/04/19 06:00
MHDA- 2011/09/01 06:00
PMCR- 2011/04/15
CRDT- 2011/04/19 06:00
PHST- 2011/02/16 00:00 [received]
PHST- 2011/04/15 00:00 [accepted]
PHST- 2011/04/19 06:00 [entrez]
PHST- 2011/04/19 06:00 [pubmed]
PHST- 2011/09/01 06:00 [medline]
PHST- 2011/04/15 00:00 [pmc-release]
AID - 1743-422X-8-172 [pii]
AID - 10.1186/1743-422X-8-172 [doi]
PST - epublish
SO  - Virol J. 2011 Apr 15;8:172. doi: 10.1186/1743-422X-8-172.