PMID- 21497661
OWN - NLM
STAT- MEDLINE
DCOM- 20120321
LR  - 20111228
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 17
IP  - 6
DP  - 2011 Dec
TI  - Microbiota profile in feces of breast- and formula-fed newborns by using 
      fluorescence in situ hybridization (FISH).
PG  - 478-82
LID - 10.1016/j.anaerobe.2011.03.009 [doi]
AB  - The development of the gut is controlled and modulated by different interacting 
      mechanisms such as, genetic endowment, intrinsic biological regulatory functions, 
      environment influences and last but no least, the diet influence. Considered 
      together with other endogenous and exogenous factors the type of feeding may 
      interfere greatly in the regulation of the intestinal microbiota. During the last 
      years molecular methods offer a complementarity to the classic culture-based 
      knowledge. FISH has been applied for molecular evaluation of the microbiota in 
      newborns delivered by vaginal delivery. Eleven probes/probe combinations for 
      specific groups of faecal bacteria were used to determine the bacterial 
      composition in faecal samples of newborns infants under different types of 
      feeding. Breast-fed infants harbor a fecal microbiota by more than two times 
      increased in numbers of Bifidobacterium cells when compared to formula-fed 
      infants. After formula-feeding, Atopobium was found in significant counts and the 
      numbers of Bifidobacterium dropped followed by increasing numbers in Bacteroides 
      population. Moreover, under formula feeding the infants microbiota was more 
      diverse.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Bezirtzoglou, Eugenia
AU  - Bezirtzoglou E
AD  - Democritus University of Thrace, Faculty of Agricultural Development, Department 
      of Food Science and Technology, Laboratory of Microbiology, Biotechnology and 
      Hygiene, GR68200-Orestiada, Greece. empezirt@agro.duth.gr
FAU - Tsiotsias, Arsenis
AU  - Tsiotsias A
FAU - Welling, Gjalt W
AU  - Welling GW
LA  - eng
PT  - Journal Article
DEP - 20110408
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Bacteria/*classification/*genetics
MH  - Bacterial Load
MH  - *Biota
MH  - Feces/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Infant Formula
MH  - Infant, Newborn
MH  - *Milk, Human
MH  - Oligonucleotide Probes/genetics
EDAT- 2011/04/19 06:00
MHDA- 2012/03/22 06:00
CRDT- 2011/04/19 06:00
PHST- 2011/01/10 00:00 [received]
PHST- 2011/03/26 00:00 [revised]
PHST- 2011/03/30 00:00 [accepted]
PHST- 2011/04/19 06:00 [entrez]
PHST- 2011/04/19 06:00 [pubmed]
PHST- 2012/03/22 06:00 [medline]
AID - S1075-9964(11)00033-3 [pii]
AID - 10.1016/j.anaerobe.2011.03.009 [doi]
PST - ppublish
SO  - Anaerobe. 2011 Dec;17(6):478-82. doi: 10.1016/j.anaerobe.2011.03.009. Epub 2011 
      Apr 8.