PMID- 21498965
OWN - NLM
STAT- MEDLINE
DCOM- 20110902
LR  - 20190727
IS  - 1349-3329 (Electronic)
IS  - 0040-8727 (Linking)
VI  - 224
IP  - 1
DP  - 2011 May
TI  - Hypoxia induces osteogenesis-related activities and expression of core binding 
      factor alpha1 in mesenchymal stem cells.
PG  - 7-12
AB  - Mesenchymal stem sells (MSCs) have received much attention in the field of bone 
      tissue engineering due to their biological capability to differentiate into 
      osteogenic lineage cells. Hypoxia-inducible factor 1alpha (HIF-1alpha) plays an 
      important role in the MSC-related bone regeneration during hypoxia, while core 
      binding factor alpha 1 (Cbfalpha1) is a transcription regulator that is involved in 
      the chondrocyte differentiation and ossification. In the present study, we 
      investigated the effects of hypoxia on biological capability of MSCs. MSCs were 
      isolated from adult rabbit bone marrow, and were cultured in vitro under normoxia 
      (air with 5% CO(2)) or hypoxia (5% CO(2) and 95% N(2)). The proliferation of 
      MSCs, alkaline phosphatase (ALP) activity, and production of collagens type I and 
      type III (Col I/III) were examined. The expression levels of HIF-1alpha and Cbfalpha1 
      were measured by real-time PCR and western blot analyses. We found that hypoxia 
      significantly induced the proliferation of MSCs and increased ALP activity and 
      the production of Col I/III. Moreover, hypoxia increased the expression of Cbfalpha1 
      mRNA after 12 h, whereas the expression of HIF-1alpha mRNA was increased after 1 h of 
      hypoxia. Knockdown of HIF-1alpha expression with a small interfering RNA 
      significantly increased the expression levels of Cbfalpha1 protein either under the 
      normoxia or hypoxia condition. Our results indicate that hypoxia enhances MSCs to 
      differentiate into osteogenic lineage cells and suggest that Cbfalpha1 may be 
      negatively regulated by HIF-1alpha.
FAU - Huang, Jiao
AU  - Huang J
AD  - Department of Periodontology, Affiliated Hospital of Stomatology, Chongqing 
      University of Medical Sciences, PR China.
FAU - Deng, Feng
AU  - Deng F
FAU - Wang, Lu
AU  - Wang L
FAU - Xiang, Xue-Rong
AU  - Xiang XR
FAU - Zhou, Wen-Wen
AU  - Zhou WW
FAU - Hu, Na
AU  - Hu N
FAU - Xu, Ling
AU  - Xu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia/genetics/physiology
MH  - Cell Proliferation
MH  - Core Binding Factor Alpha 1 Subunit/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - *Osteogenesis/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
EDAT- 2011/04/19 06:00
MHDA- 2011/09/03 06:00
CRDT- 2011/04/19 06:00
PHST- 2011/04/19 06:00 [entrez]
PHST- 2011/04/19 06:00 [pubmed]
PHST- 2011/09/03 06:00 [medline]
AID - JST.JSTAGE/tjem/224.7 [pii]
AID - 10.1620/tjem.224.7 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 2011 May;224(1):7-12. doi: 10.1620/tjem.224.7.