PMID- 21499738
OWN - NLM
STAT- MEDLINE
DCOM- 20120201
LR  - 20211020
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 36
IP  - 8
DP  - 2011 Aug
TI  - Age and visual experience-dependent expression of NMDAR1 splice variants in rat 
      retina.
PG  - 1417-25
LID - 10.1007/s11064-011-0467-5 [doi]
AB  - The N-methyl-D-aspartate receptor (NMDAR) is a key molecule mediating brain 
      plasticity related processes. Knowing that alternative splicing of the NMDAR1 
      (NR1) subunit offers molecular diversity to NMDAR, controls the forward 
      trafficking of the NR1 protein and is important for placing NMDA receptors at 
      synapses, we investigated herein the postnatal developmental expression and the 
      influence of visual deprivation on NR1 subunit splice variants in rat retina. 
      Real-time PCR was performed using oligonucleotide primers specific for N- 
      terminal (NR1a, NR1b) and C-terminal splice variants (NR1-1, NR1-2, NR1-3, 
      NR1-4). The developmental profiles of mRNA expression levels of all NR1 isoforms 
      peaked at the end of the third week. Dark rearing led to reductions in both N- 
      and C-terminal NR1 variants in several developmental ages and a significant 
      interaction between age and visual experience was observed for NR1a, NR1-2 and 
      NR1-4 expression. Our results have demonstrated a developmental and visual 
      experience-dependent regulation of NR1 splicing in rat retina.
FAU - Manta, Georgia
AU  - Manta G
AD  - Department of Physiology, School of Medicine, University of Patras, 26500, 
      Patras, Greece.
FAU - Spathis, Athanasios D
AU  - Spathis AD
FAU - Taraviras, Stavros
AU  - Taraviras S
FAU - Kouvelas, Elias D
AU  - Kouvelas ED
FAU - Mitsacos, Adamantia
AU  - Mitsacos A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110416
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (NMDA receptor A1)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Aging/*physiology
MH  - *Alternative Splicing
MH  - Animals
MH  - Darkness
MH  - Protein Isoforms/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Retina/*metabolism
MH  - Vision, Ocular/*physiology
EDAT- 2011/04/19 06:00
MHDA- 2012/02/02 06:00
CRDT- 2011/04/19 06:00
PHST- 2011/03/30 00:00 [accepted]
PHST- 2011/04/19 06:00 [entrez]
PHST- 2011/04/19 06:00 [pubmed]
PHST- 2012/02/02 06:00 [medline]
AID - 10.1007/s11064-011-0467-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2011 Aug;36(8):1417-25. doi: 10.1007/s11064-011-0467-5. Epub 2011 
      Apr 16.