PMID- 21502801
OWN - NLM
STAT- MEDLINE
DCOM- 20130329
LR  - 20131121
IS  - 1720-8319 (Electronic)
IS  - 1594-0667 (Linking)
VI  - 24
IP  - 2
DP  - 2012 Apr
TI  - The effects of caloric restriction and a high-fat diet on ovarian lifespan and 
      the expression of SIRT1 and SIRT6 proteins in rats.
PG  - 125-33
LID - 10.3275/7660 [doi]
AB  - BACKGROUND AND AIMS: Caloric restriction (CR) extends mammals' lifespans and 
      suppresses ovary development. Sirtuins are involved in these mechanisms. If, and 
      to what extent CR affects ovarian lifespan and follicle development is largely 
      unknown. We investigated the effects of moderate and severe caloric restriction 
      compared with a high-fat dietary regimen on ovarian follicle reserves in rats. 
      METHODS: Female Sprague-Dawley rats (n=48) randomly divided into four groups 
      including normal control (NC), 25% caloric restriction (MCR), 45% CR (SCR) and 
      high-fat diet (HF) were maintained on these regimens for 2 months. RESULTS: 
      Histological analysis showed that both the 25 and 45% CR rats had a significantly 
      higher percentage of primordial follicles and a larger number of healthy 
      follicles than the NC rats, whereas the HF rats did not differ significantly from 
      the NC rats. Immunohistochemical analysis revealed that SIRT1 and SIRT6 proteins 
      were present in the nucleus and cytoplasm of the oocytes. The 25% CR diet 
      increased the expression of both SIRT1 and SIRT6 in the ovary, whereas the 45% CR 
      and HF diets caused a decrease in SIRT1 expression. The level of SIRT6 protein 
      did not change with the 45% CR diet, and it appeared slightly lower in the HF 
      than in the NC groups. CONCLUSIONS: Caloric restriction may inhibit the 
      transition from primordial to developing follicles and extend the entire growth 
      phase of a follicle to preserve the reserve of germ cells. SIRT1 and SIRT6 are 
      both associated with these effects.
FAU - Luo, Li-Li
AU  - Luo LL
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of Shantou 
      University Medical College, Shantou, Guangdong 515041, PR China. 
      luolili6606@yahoo.com
FAU - Chen, Xiao-Chun
AU  - Chen XC
FAU - Fu, Yu-Cai
AU  - Fu YC
FAU - Xu, Jin-Jie
AU  - Xu JJ
FAU - Li, Li
AU  - Li L
FAU - Lin, Xuan-Hao
AU  - Lin XH
FAU - Xiang, Yan-Fang
AU  - Xiang YF
FAU - Zhang, Xing-Mei
AU  - Zhang XM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110414
PL  - Germany
TA  - Aging Clin Exp Res
JT  - Aging clinical and experimental research
JID - 101132995
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - EC 3.5.1.- (Sirtuins)
RN  - EC 3.5.1.- (sirtuin 6, rat)
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - *Caloric Restriction
MH  - Cholesterol/blood
MH  - *Diet, High-Fat
MH  - Energy Intake/physiology
MH  - Female
MH  - Ovarian Follicle/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sirtuin 1/*biosynthesis/metabolism
MH  - Sirtuins/*biosynthesis/metabolism
MH  - Triglycerides/blood
EDAT- 2011/04/20 06:00
MHDA- 2013/03/30 06:00
CRDT- 2011/04/20 06:00
PHST- 2011/04/20 06:00 [entrez]
PHST- 2011/04/20 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 7660 [pii]
AID - 10.3275/7660 [doi]
PST - ppublish
SO  - Aging Clin Exp Res. 2012 Apr;24(2):125-33. doi: 10.3275/7660. Epub 2011 Apr 14.