PMID- 21503112
OWN - NLM
STAT- MEDLINE
DCOM- 20120503
LR  - 20171116
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 43
IP  - 2
DP  - 2011 Apr 18
TI  - [Detection of human epidermal growth factor receptor 2 gene in invasive breast 
      cancer by fluorescence in situ hybridization and research of its association with 
      tumor characteristics].
PG  - 199-203
AB  - OBJECTIVE: To investigate the concordance of detecting human epidermal growth 
      factor receptor 2 (HER-2) status of patients with invasive breast cancer by 
      fluorescence in situ hybridization (FISH) for gene amplification and 
      immunohistochemistry (IHC) for protein overexpression and to evaluate the 
      association between tumour characteristics and HER-2 gene amplification. METHODS: 
      The HER-2 status was evaluated in 41 invasive ductal breast carcinomas by both 
      FISH and IHC. The associations between HER-2 gene amplification and other 
      clinicopathological factors, tumour grade, estrogen receptor (ER), tumor emboli, 
      lymph node status, were evaluated using the chi-square test. RESULTS: HER-2 
      amplifications by FISH were seen in 15 patients, including 8 IHC+++ patients 
      (88.89%, 8/9 of all+++ cases), 6 IHC++ patients (42.68%, 6/14 of all++ cases), 1 
      IHC+ patient (8.33%, 1/12 of all+ cases) and none IHC negative patient (0%, 0/6 
      of all negative cases). ER negative or weak positive breast cancers were more 
      often HER-2 FISH positive than ER strong positive cancers (P=0.018). HER-2 
      amplifications were more often negative in tumor emboli negative or lymph node 
      negative patients than in tumor emboli positive or lymph node positive patients 
      (P=0.000, P=0.025). There was no statistical difference in HER-2 gene 
      amplification among different grades of tumors(P=0.095). CONCLUSION: HER-2 
      amplification in breast cancer can be determined by FISH accurately. ER is 
      negatively associated with HER-2 amplification, whereas tumor emboli and lymph 
      node status are positively associated with HER-2.
FAU - Su, Jing
AU  - Su J
AD  - Department of Pathology, Peking University School of Basic Medical Sciences, 
      Beijing 100191, China.
FAU - Yang, Yu
AU  - Yang Y
FAU - Ma, Xiao-long
AU  - Ma XL
FAU - You, Jiang-feng
AU  - You JF
FAU - DU, Juan
AU  - DU J
FAU - Liu, Jian-ying
AU  - Liu JY
FAU - Zheng, Jie
AU  - Zheng J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (Receptors, Estrogen)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics/*pathology
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Invasiveness
MH  - Receptor, ErbB-2/*genetics
MH  - Receptors, Estrogen/metabolism
EDAT- 2011/04/20 06:00
MHDA- 2012/05/04 06:00
CRDT- 2011/04/20 06:00
PHST- 2011/04/20 06:00 [entrez]
PHST- 2011/04/20 06:00 [pubmed]
PHST- 2012/05/04 06:00 [medline]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2011 Apr 18;43(2):199-203.