PMID- 21503399
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20191027
IS  - 1678-4391 (Electronic)
IS  - 1413-8670 (Linking)
VI  - 15
IP  - 2
DP  - 2011 Mar-Apr
TI  - Enterotoxins, colonization factors, serotypes and antimicrobial resistance of 
      enterotoxigenic Escherichia coli (ETEC) strains isolated from hospitalized 
      children with diarrhea in Bolivia.
PG  - 132-7
AB  - Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of 
      bacterial diarrhoea among children in Asia, Africa and Latin America but less 
      investigated in Bolivia. OBJECTIVE: To determine the relation between 
      enterotoxins, CFs and serotypes as well as the antimicrobial resistance patterns 
      in a set of ETEC isolates collected from hospitalized children with acute 
      diarrhea. In the present study we characterized 43 ETEC strains isolated from 
      2002 to 2006 from hospitalized children (0-5 years) with acute diarrhea in 
      Bolivia. The strains were analyzed for heat-labile (LT) and heat-stable (ST) 
      enterotoxins and colonization factor (CF) profiles, as well as for serogroups and 
      antimicrobial resistance using phenotypic (ELISA, dot blot, slide agglutination 
      and disc diffusion) and genotypic (Multiplex PCR) methods. Among the ETEC 
      isolates tested, 30 were positive for LT, 3 for STh and 10 for LT/STh. Sixty-five 
      percent (28/43) of the strains expressed one or more CF. The most common CFs were 
      CS17 (n = 8) and CFA/I (n = 8). The phenotypical and genotypical results for 
      toxins and CFs were congruent except for CS21 that was amplified in 10 of the 
      strains by multiplex PCR, but CS21 pili was only detected phenotypically in four 
      of these strains. The ETEC strains had diverse O and H antigens and the most 
      common types were O8:H9 LT CS17 (n = 6; 14%) and O78:HNM LT-ST CFA/I (n = 4; 9%). 
      The analysis of antibiotic resistance showed that 67% (n = 29/43) of the strains 
      were resistant to one or several of the antimicrobial agents tested. Presence of 
      CFs was associated with antibiotic resistance. CONCLUSION: The most common toxin 
      profile was LT 70%, LT/STh 23% and STh 7%. High antimicrobial resistance to 
      ampicillin among serogroups O6, O8 and O78 were the most common.
FAU - Rodas, Claudia
AU  - Rodas C
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, Sweden.
FAU - Mamani, Rosalia
AU  - Mamani R
FAU - Blanco, Jorge
AU  - Blanco J
FAU - Blanco, Jesus Eulogio
AU  - Blanco JE
FAU - Wiklund, Gudrun
AU  - Wiklund G
FAU - Svennerholm, Ann-Mari
AU  - Svennerholm AM
FAU - Sjoling, Asa
AU  - Sjoling A
FAU - Iniguez, Volga
AU  - Iniguez V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz J Infect Dis
JT  - The Brazilian journal of infectious diseases : an official publication of the 
      Brazilian Society of Infectious Diseases
JID - 9812937
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bolivia
MH  - Child, Preschool
MH  - Diarrhea/*microbiology
MH  - *Enterotoxigenic Escherichia coli/chemistry/drug effects/genetics
MH  - Enterotoxins/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Escherichia coli Infections/complications/*microbiology
MH  - Genotype
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Phenotype
MH  - Serotyping
EDAT- 2011/04/20 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/04/20 06:00
PHST- 2010/08/11 00:00 [received]
PHST- 2010/09/27 00:00 [accepted]
PHST- 2011/04/20 06:00 [entrez]
PHST- 2011/04/20 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - S1413-8670(11)70158-1 [pii]
AID - 10.1016/s1413-8670(11)70158-1 [doi]
PST - ppublish
SO  - Braz J Infect Dis. 2011 Mar-Apr;15(2):132-7. doi: 10.1016/s1413-8670(11)70158-1.