PMID- 21504583
OWN - NLM
STAT- MEDLINE
DCOM- 20110907
LR  - 20211020
IS  - 1476-511X (Electronic)
IS  - 1476-511X (Linking)
VI  - 10
DP  - 2011 Apr 19
TI  - High fat diet-induced non alcoholic fatty liver disease in rats is associated 
      with hyperhomocysteinemia caused by down regulation of the transsulphuration 
      pathway.
PG  - 60
LID - 10.1186/1476-511X-10-60 [doi]
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) causes increased oxidative stress and is 
      an independent risk factor for cardiovascular disease. Oxidative stress is now 
      believed to be a major contributory factor in the development of non alcoholic 
      fatty liver disease, the most common liver disorder worldwide. In this study, the 
      changes which occur in homocysteine (Hcy) metabolism in high fat-diet induced non 
      alcoholic fatty liver disease (NAFLD) in rats were investigated. METHODS AND 
      RESULTS: After feeding rats a standard low fat diet (control) or a high fat diet 
      (57% metabolisable energy as fat) for 18 weeks, the concentration of homocysteine 
      in the plasma was significantly raised while that of cysteine was lowered in the 
      high fat as compared to the control diet fed animals. The hepatic activities of 
      cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CGS), the enzymes 
      responsible for the breakdown of homocysteine to cysteine via the 
      transsulphuration pathway in the liver, were also significantly reduced in the 
      high fat-fed group. CONCLUSIONS: These results indicate that high fat 
      diet-induced NAFLD in rats is associated with increased plasma Hcy levels caused 
      by down-regulation of hepatic CBS and CGL activity. Thus, HHcy occurs at an early 
      stage in high fat diet-induced NAFLD and is likely to contribute to the increased 
      risk of cardiovascular disease associated with the condition.
FAU - Bravo, Elena
AU  - Bravo E
AD  - Department of Cellular Biology and Neuroscience, Istituto Superiore Sanita, Rome, 
      Italy. elena.bravo@iss.it
FAU - Palleschi, Simonetta
AU  - Palleschi S
FAU - Aspichueta, Patricia
AU  - Aspichueta P
FAU - Buque, Xabier
AU  - Buque X
FAU - Rossi, Barbara
AU  - Rossi B
FAU - Cano, Ainara
AU  - Cano A
FAU - Napolitano, Mariarosaria
AU  - Napolitano M
FAU - Ochoa, Begona
AU  - Ochoa B
FAU - Botham, Kathleen M
AU  - Botham KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110419
PL  - England
TA  - Lipids Health Dis
JT  - Lipids in health and disease
JID - 101147696
RN  - 0 (Dietary Fats)
RN  - 0 (Insulin)
RN  - 0 (Triglycerides)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
SB  - IM
MH  - Animals
MH  - Cystathionine beta-Synthase/metabolism
MH  - Cystathionine gamma-Lyase/metabolism
MH  - *Dietary Fats
MH  - Down-Regulation
MH  - Fatty Liver/*etiology/metabolism
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/*etiology/metabolism
MH  - Insulin/blood
MH  - Liver/metabolism
MH  - Male
MH  - *Metabolic Networks and Pathways
MH  - Methyltransferases/genetics/metabolism
MH  - Non-alcoholic Fatty Liver Disease
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription, Genetic
MH  - Triglycerides/metabolism
PMC - PMC3096990
EDAT- 2011/04/21 06:00
MHDA- 2011/09/08 06:00
PMCR- 2011/04/19
CRDT- 2011/04/21 06:00
PHST- 2011/03/03 00:00 [received]
PHST- 2011/04/19 00:00 [accepted]
PHST- 2011/04/21 06:00 [entrez]
PHST- 2011/04/21 06:00 [pubmed]
PHST- 2011/09/08 06:00 [medline]
PHST- 2011/04/19 00:00 [pmc-release]
AID - 1476-511X-10-60 [pii]
AID - 10.1186/1476-511X-10-60 [doi]
PST - epublish
SO  - Lipids Health Dis. 2011 Apr 19;10:60. doi: 10.1186/1476-511X-10-60.