PMID- 21504994
OWN - NLM
STAT- MEDLINE
DCOM- 20111019
LR  - 20110628
IS  - 1758-1117 (Electronic)
IS  - 0023-6772 (Linking)
VI  - 45
IP  - 3
DP  - 2011 Jul
TI  - The effect of standard chow and reduced hexokinase II on growth, cardiac and 
      skeletal muscle hexokinase and low-flow cardiac ischaemia-reperfusion injury.
PG  - 160-6
LID - 10.1258/la.2011.010096 [doi]
AB  - In the present study, we examined whether standard chow (SDS versus Purina 5001; 
      both low fat, high carbohydrate) and reductions in hexokinase (HK) II (wild-type 
      versus HKII(+/-) mice) affect (1) growth parameters, (2) HK levels in cardiac and 
      skeletal muscle and (3) low-flow cardiac ischaemia-reperfusion (IR) injury. Total 
      HK activity and HKI and HKII expressions were determined, and low-flow IR injury 
      was examined in isolated hearts subjected to 40 min 5% low-flow ischaemia and 120 
      min reperfusion. Standard chow, but not HKII reductions, significantly affected 
      body weight, heart weight and cardiac hypertrophy. Both standard chow and reduced 
      HKII diminished total cardiac and skeletal muscle HK activity. For the heart, the 
      Purina chow-induced decrease in total HK activity was through decreases in HKI 
      expression, whereas for skeletal muscle post-translational mechanisms are 
      suggested. Both standard chow and reduced HKII demonstrated a non-significant 
      trend for affecting cardiac IR damage. However, the low-flow ischaemia model was 
      associated with mild sublethal injury only ( approximately 1% cell death). In conclusion, 
      standard chow affects body weight, heart weight and HK activity and HKI 
      expression in the heart, without altering HKII expression. This implicates 
      standard chow as an important factor in genomic, physiological research models 
      and demonstrates that large differences in fat or carbohydrates in the diet are 
      not necessary to affect growth. In a cardiac low-flow IR model, resulting in only 
      mild injury, standard chow or reduced HKII does not significantly affect IR 
      damage.
FAU - Smeele, Kirsten M
AU  - Smeele KM
AD  - Laboratory of Experimental Intensive Care and Anesthesiology (LEICA), Department 
      of Anesthesiology, Academic Medical Center (AMC), University of Amsterdam, 
      Amsterdam, The Netherlands.
FAU - ter Horst, Leontien H
AU  - ter Horst LH
FAU - Koeman, Anneke
AU  - Koeman A
FAU - Heikkinen, Sami
AU  - Heikkinen S
FAU - Laakso, Markku
AU  - Laakso M
FAU - Weber, Nina C
AU  - Weber NC
FAU - Hollmann, Markus W
AU  - Hollmann MW
FAU - Zuurbier, Coert J
AU  - Zuurbier CJ
LA  - eng
PT  - Journal Article
DEP - 20110419
PL  - England
TA  - Lab Anim
JT  - Laboratory animals
JID - 0112725
RN  - EC 2.7.1.1 (HK1 protein, mouse)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Cardiomegaly/enzymology/genetics/metabolism
MH  - Diet/veterinary
MH  - Female
MH  - Hexokinase/genetics/*metabolism
MH  - Male
MH  - Mice/genetics/growth & development/*physiology
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*enzymology/metabolism
MH  - Myocardium/*enzymology/metabolism
MH  - Organ Size
MH  - Reperfusion Injury/enzymology/genetics/*metabolism
EDAT- 2011/04/21 06:00
MHDA- 2011/10/20 06:00
CRDT- 2011/04/21 06:00
PHST- 2011/04/21 06:00 [entrez]
PHST- 2011/04/21 06:00 [pubmed]
PHST- 2011/10/20 06:00 [medline]
AID - la.2011.010096 [pii]
AID - 10.1258/la.2011.010096 [doi]
PST - ppublish
SO  - Lab Anim. 2011 Jul;45(3):160-6. doi: 10.1258/la.2011.010096. Epub 2011 Apr 19.