PMID- 21505327
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20110425
IS  - 1473-6322 (Electronic)
IS  - 1473-6322 (Linking)
VI  - 11
IP  - 3
DP  - 2011 Jun
TI  - Molecular diagnosis of cow's milk allergy.
PG  - 216-21
LID - 10.1097/ACI.0b013e32834694ef [doi]
AB  - PURPOSE OF REVIEW: To identify and discuss studies on the molecular diagnosis of 
      cow's milk allergy (CMA) with a view to update allergists since a general review 
      of the methodology in 2006. RECENT FINDINGS: Seven basic research studies 
      reporting the use of component-resolved diagnostics in CMA were found. All 
      studies were on children positively reacting to a formal challenge with cow's 
      milk. Six studies used natural allergens and three used recombinant milk 
      proteins. Microarray platforms were customized and, thus, differed across 
      studies. Three studies assessed the association between molecular-scale patterns 
      and different presentations of the condition, that is the association between 
      anaphylaxis, gastrointestinal symptoms and other severe phenotypes and the 
      pattern of protein sensitization. Two studies assessed the association between 
      positive oral food challenge and the persistence of milk allergy over time. 
      Protein profiling could be useful to indicate appropriate specific immunotherapy. 
      SUMMARY: Accurate diagnosis of CMA is challenging and essential. The 
      determination of the immunoglobulin E (IgE)-mediated response to sequenced and 
      characterized allergens may be more useful in predicting the presence and 
      severity of clinical allergy than the currently used skin or blood tests 
      performed with whole extracts. However, as component recognition pattern 
      heterogeneity is observed in different areas, further clinical studies are 
      essential to correlate useful molecular diagnostics and biological markers with 
      disease and patient profiles. Until such markers are found and validated in 
      different age groups, oral food challenge remains the reference standard for the 
      diagnosis of CMA.
FAU - Fiocchi, Alessandro
AU  - Fiocchi A
AD  - Paediatric Division, Department of Child and Maternal Medicine, University of 
      Milan Medical School at Melloni Hospital, Milan, Italy. allerg@tin.it
FAU - Bouygue, Gabriel R
AU  - Bouygue GR
FAU - Albarini, Marco
AU  - Albarini M
FAU - Restani, Patrizia
AU  - Restani P
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Curr Opin Allergy Clin Immunol
JT  - Current opinion in allergy and clinical immunology
JID - 100936359
RN  - 0 (Allergens)
RN  - 0 (Milk Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Cattle
MH  - Child
MH  - *Desensitization, Immunologic
MH  - Disease Progression
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - *Microarray Analysis
MH  - Milk Hypersensitivity/*diagnosis/physiopathology/therapy
MH  - Milk Proteins/immunology
MH  - Pathology, Molecular/methods
MH  - Prognosis
MH  - Recombinant Proteins/immunology
MH  - Skin Tests
EDAT- 2011/04/21 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/04/21 06:00
PHST- 2011/04/21 06:00 [entrez]
PHST- 2011/04/21 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - 10.1097/ACI.0b013e32834694ef [doi]
PST - ppublish
SO  - Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21. doi: 
      10.1097/ACI.0b013e32834694ef.