PMID- 21512117
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20131121
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 89
IP  - 9
DP  - 2011 Sep
TI  - Functional genomics of the muscle response to restraint and transport in 
      chickens.
PG  - 2717-30
LID - 10.2527/jas.2010-3288 [doi]
AB  - In the present study, we used global approaches (proteomics, transcriptomics, and 
      metabolomics) to assess the molecular basis of the muscle response to stress in 
      chickens. A restraint test, combined with transport for 2 h (RT test) was chosen 
      as the potentially stressful situation. Chickens (6 wk old) were either 
      nontreated (control chickens) or submitted to the RT test (treated chickens). The 
      RT test induced a 6-fold increase in corticosterone concentrations, suggesting 
      hypothalamic-pituitary-adrenal axis activation. The RT test decreased the 
      relative abundance of several hexose phosphates [glucose-1-P (G1P), glucose-6-P 
      (G6P), fructose-6-P (F6P), and mannose-6-P (M6P)] in thigh muscle. In addition, 
      55 transcripts, among which 39 corresponded to unique annotated genes, were 
      significantly up- (12 genes) or downregulated (27 genes) by treatment. Similarly, 
      45 proteic spots, among which 29 corresponded to unique annotated proteins, were 
      overexpressed (11 proteins), underexpressed (14 proteins), or only expressed in 
      treated chickens. Integrative analysis of differentially expressed genes and 
      proteins showed that most transcripts and proteins belong to 2 networks whose 
      genes were mainly related with cytoskeleton structure or carbohydrate metabolism. 
      Whereas the decrease in energetic metabolites suggested an activation of 
      glycogenolysis and glycolysis in response to the RT test, the reduced expression 
      of genes and proteins involved in these pathways suggested the opposite. We 
      hypothesized that the prolonged RT test resulted in a repression of 
      glycogenolysis and glycolysis in thigh muscle of chickens. The down-expression of 
      genes and proteins involved in the formation of fiber stress after the RT test 
      suggests a reinforcement of myofibrils in response to stress.
FAU - Hazard, D
AU  - Hazard D
AD  - Universite de Toulouse, INPT ENSAT, UMR1289 Tissus Animaux Nutrition Digestion 
      Ecosysteme et Metabolisme, F-31326 Castanet-Tolosan, France.
FAU - Fernandez, X
AU  - Fernandez X
FAU - Pinguet, J
AU  - Pinguet J
FAU - Chambon, C
AU  - Chambon C
FAU - Letisse, F
AU  - Letisse F
FAU - Portais, J-C
AU  - Portais JC
FAU - Wadih-Moussa, Z
AU  - Wadih-Moussa Z
FAU - Remignon, H
AU  - Remignon H
FAU - Molette, C
AU  - Molette C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110421
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Lactates)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Chickens/*genetics/metabolism/physiology
MH  - Corticosterone/blood
MH  - Electrophoresis, Gel, Two-Dimensional/veterinary
MH  - Genomics
MH  - Glucose/analysis
MH  - Glycogen/analysis
MH  - Lactates/analysis
MH  - Metabolomics
MH  - Muscle, Skeletal/chemistry/metabolism/*physiology
MH  - Oligonucleotide Array Sequence Analysis/veterinary
MH  - Proteomics
MH  - *Quantitative Trait, Heritable
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Restraint, Physical/veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Stress, Physiological/*genetics
MH  - Transportation
EDAT- 2011/04/23 06:00
MHDA- 2011/12/15 06:00
CRDT- 2011/04/23 06:00
PHST- 2011/04/23 06:00 [entrez]
PHST- 2011/04/23 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
AID - jas.2010-3288 [pii]
AID - 10.2527/jas.2010-3288 [doi]
PST - ppublish
SO  - J Anim Sci. 2011 Sep;89(9):2717-30. doi: 10.2527/jas.2010-3288. Epub 2011 Apr 21.