PMID- 21513759
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20110516
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 72
IP  - 6
DP  - 2011 Jun
TI  - Tag-single nucleotide polymorphism-based human leukocyte antigen genotyping in 
      celiac disease patients from northeastern Italy.
PG  - 499-502
LID - 10.1016/j.humimm.2011.03.008 [doi]
AB  - We genotyped celiac disease (CD)-associated haplotypes DQ2.5, DQ8, DQ2.2, and DQ7 
      in 1005 CD patients from North Eastern Italy using a Tag-single nucleotide 
      polymorphism (SNPs) approach and real time PCR platform, checking the accuracy 
      and reliability of the method and comparing it to traditional PCR-SSP. Only 14 of 
      2010 chromosomes analyzed (0.7%) showed discrepancies between the Tag-SNPs 
      real-time polymerase chain reaction (PCR) method and the PCR-single-strand 
      polymorphism (SSP) technique, indicating a high sensitivity and specificity 
      (ranging from 0.987 to 1 and from 0.998 to 0.999, respectively) for tagging with 
      respect to corresponding human leukocyte antigen (HLA) alleles identified by 
      PCR-SSP. Moreover, the overall cost of the Tag-SNPs HLA typing method was low (3 
      to 4 euro/sample instead of 35 to 70 euro/sample with commercial kits), making it 
      suitable for mass screenings. Hence, we believe that the Tag-SNPs HLA typing 
      could be used to complement or replace classic HLA typing in at high-risk groups, 
      for research purposes and eventually in population screening programs.
CI  - Copyright (c) 2011 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Vatta, Serena
AU  - Vatta S
AD  - Laboratory of Immuno-Pathology, Istituto di Ricovero e Cura a Carattere 
      Scientifico Burlo Garofolo, Trieste, Italy.
FAU - Fabris, Annalisa
AU  - Fabris A
FAU - Segat, Ludovica
AU  - Segat L
FAU - Not, Tarcisio
AU  - Not T
FAU - Crovella, Sergio
AU  - Crovella S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110408
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Celiac Disease/*diagnosis/genetics/immunology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Italy
MH  - Male
MH  - Mass Screening
MH  - Middle Aged
MH  - *Polymerase Chain Reaction/methods
MH  - Polymorphism, Single Nucleotide
MH  - Reproducibility of Results
MH  - *Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
EDAT- 2011/04/26 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/04/26 06:00
PHST- 2011/01/10 00:00 [received]
PHST- 2011/03/15 00:00 [revised]
PHST- 2011/03/31 00:00 [accepted]
PHST- 2011/04/26 06:00 [entrez]
PHST- 2011/04/26 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - S0198-8859(11)00061-9 [pii]
AID - 10.1016/j.humimm.2011.03.008 [doi]
PST - ppublish
SO  - Hum Immunol. 2011 Jun;72(6):499-502. doi: 10.1016/j.humimm.2011.03.008. Epub 2011 
      Apr 8.