PMID- 21513977
OWN - NLM
STAT- MEDLINE
DCOM- 20110912
LR  - 20181201
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 32
IP  - 6
DP  - 2011 Jun
TI  - Effect of the maternofetal and milk transfer of the anti-epidermal growth factor 
      receptor monoclonal antibody 7A7 in mice.
PG  - 470-4
LID - 10.1016/j.placenta.2011.03.011 [doi]
AB  - OBJECTIVES: To study the maternofetal and milk transfer of an anti-epidermal 
      growth factor receptor (EGFR) monoclonal antibody (MAb) and its effects on 
      conceptus, we administered to pregnant and lactating dams the murine anti-EGFR 
      MAb 7A7 in an autologus model. STUDY DESIGN: For determining the embryo-fetal 
      toxicity, 7A7 at gestational days (GD) 6, 8, 12 and 14 were intravenously 
      administered. Clinical signs and body weights were recorded. On GD 18 pregnant 
      mice were euthanized and the alive and dead fetuses were examined. For measuring 
      the maternofetal transfer mice were dosed on GD 14 with (125)I-7A7, after 24 h, 
      mice were euthanized and main maternal organs and fetuses were counted separately 
      for radioactivity. For studying the MAb transferred throughout the milk, 
      lactating dams were intravenously dosed on lactation day (LD) 2 with (125)I-7A7. 
      Blood samples were obtained from dams at different times post. One lactating pup 
      from each dam was also euthanized at different times, and their blood and gastric 
      milk were removed and the radioactivities measured. RESULTS: The administration 
      of the 7A7 did not elicit toxicity to adult pregnant mice nevertheless; there was 
      evidence of embryo-fetal toxicity in the 7A7 group characterized by a decrease in 
      litters' body weights and head deformities. The maternofetal transfer of 
      (125)I-7A7 antibody on GD 15 was only of a 4%. CONCLUSION: Results suggest that 
      7A7 crosses placenta and it is transferred in a superior quantity to pups through 
      the milk and that anti-EGFR MAbs have a potential toxic effect to fetuses.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Gonzalez, J E
AU  - Gonzalez JE
AD  - Laboratorio de Radiobiologia, Centro de Proteccion e Higiene de las Radiaciones, 
      La Habana, Cuba.
FAU - Leon, M
AU  - Leon M
FAU - Hernandez, I
AU  - Hernandez I
FAU - Garrido, G
AU  - Garrido G
FAU - Casaco, A
AU  - Casaco A
LA  - eng
PT  - Journal Article
DEP - 20110421
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Antibodies, Monoclonal)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - ErbB Receptors/*immunology
MH  - Female
MH  - Lactation/*immunology
MH  - Male
MH  - Mice
MH  - Pregnancy
EDAT- 2011/04/26 06:00
MHDA- 2011/09/13 06:00
CRDT- 2011/04/26 06:00
PHST- 2011/03/23 00:00 [received]
PHST- 2011/03/25 00:00 [accepted]
PHST- 2011/04/26 06:00 [entrez]
PHST- 2011/04/26 06:00 [pubmed]
PHST- 2011/09/13 06:00 [medline]
AID - S0143-4004(11)00124-X [pii]
AID - 10.1016/j.placenta.2011.03.011 [doi]
PST - ppublish
SO  - Placenta. 2011 Jun;32(6):470-4. doi: 10.1016/j.placenta.2011.03.011. Epub 2011 
      Apr 21.