PMID- 21515256
OWN - NLM
STAT- MEDLINE
DCOM- 20110906
LR  - 20110517
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 660
IP  - 2-3
DP  - 2011 Jun 25
TI  - Effect of brain-derived neurotrophic factor on activity-regulated 
      cytoskeleton-associated protein gene expression in primary frontal cortical 
      neurons. Comparison with NMDA and AMPA.
PG  - 351-7
LID - 10.1016/j.ejphar.2011.03.055 [doi]
AB  - The effect of brain-derived neurotrophic factor (BDNF) on activity-regulated 
      cytoskeleton-associated protein (Arc) mRNA levels in primary neuronal cultures of 
      rat frontal cortex was characterized pharmacologically and compared to the effect 
      on expression of c-fos, bdnf, neuritin, cox-2 as examples of other immediate 
      early genes. BDNF induced a very strong increase (around 100 fold) in Arc mRNA 
      and the maximal effect seen at 25 ng/ml. The effect was dose-dependent with EC50 
      around 1.6 ng/ml. The time profile revealed a significant effect after 25 min. 
      BDNF also increased levels of c-Fos, neuritin and BDNF mRNA, but not COX-2 mRNA. 
      The pharmacological profile of NMDA and AMPA-induced arc gene expression in 
      frontal cortical neurons was compared to BDNF. NMDA and AMPA increased Arc mRNA 
      but their maximal effect did not exceed 20-fold. The effect of AMPA was 
      completely blocked by the NMDA receptor antagonist MK-801. Further, the relative 
      amount of Arc mRNA compared to c-Fos mRNA was higher for BDNF, equal for NMDA and 
      lower for AMPA. These results demonstrate BDNF to be a highly potent and 
      efficient inducer of arc gene expression in vitro, emphasizing the role of this 
      growth factor in synaptic plasticity in the frontal cortex.
CI  - Copyright (c) 2011. Published by Elsevier B.V.
FAU - El-Sayed, Mona
AU  - El-Sayed M
AD  - Neurobiology Research Unit, Department of Nephrology and Endocrinology, 
      University Hospital Rigshospitalet, Copenhagen, Denmark.
FAU - Hofman-Bang, Jacob
AU  - Hofman-Bang J
FAU - Mikkelsen, Jens D
AU  - Mikkelsen JD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110419
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cytoskeletal Proteins/*genetics
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Frontal Lobe/*cytology
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - N-Methylaspartate/*pharmacology
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurons/*drug effects/metabolism
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, AMPA/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Time
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/*pharmacology
EDAT- 2011/04/26 06:00
MHDA- 2011/09/07 06:00
CRDT- 2011/04/26 06:00
PHST- 2010/08/03 00:00 [received]
PHST- 2011/03/09 00:00 [revised]
PHST- 2011/03/28 00:00 [accepted]
PHST- 2011/04/26 06:00 [entrez]
PHST- 2011/04/26 06:00 [pubmed]
PHST- 2011/09/07 06:00 [medline]
AID - S0014-2999(11)00402-X [pii]
AID - 10.1016/j.ejphar.2011.03.055 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2011 Jun 25;660(2-3):351-7. doi: 10.1016/j.ejphar.2011.03.055. 
      Epub 2011 Apr 19.