PMID- 21516418
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR  - 20110425
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 731
DP  - 2011
TI  - Molecular breakpoint analysis of chromosome translocations in cancer cell lines 
      by Long Distance Inverse-PCR.
PG  - 321-32
LID - 10.1007/978-1-61779-080-5_26 [doi]
AB  - With conventional cytogenetic screening by fluorescence in situ hybridization 
      (FISH) using genomic tilepath clones, identification of genes in oncogenic 
      chromosome translocations is often laborious, notably if the region of interest 
      is gene-dense. Conventional molecular methods for partner identification may also 
      suffer severe limitations; for instance, genomic PCR screening requires prior 
      knowledge of both sets of breakpoints, while rapid amplification of cDNA ends 
      (RACE) is not only limited to translocations causing mRNA fusion, but also fails 
      to provide potentially relevant breakpoint data. With Long Distance Inverse 
      (LDI)-PCR, however, it is theoretically possible to identify unknown 
      translocation partners and to map the breakpoints down to the base pair level. 
      Implementing LDI-PCR only requires approximate sequence information on one 
      partner, rendering it ideal for use in combination with frontline FISH analysis. 
      The protocol described here has been tuned for use by those wishing to identify 
      new cancer genes in tumor cell lines.
FAU - Schneider, Bjorn
AU  - Schneider B
AD  - DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, 
      Germany. Bjoern.Schneider@dsmz.de
FAU - Drexler, Hans G
AU  - Drexler HG
FAU - Macleod, Roderick A F
AU  - Macleod RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - *Chromosome Breakpoints
MH  - Electrophoresis, Agar Gel
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Translocation, Genetic/*genetics
EDAT- 2011/04/26 06:00
MHDA- 2011/08/24 06:00
CRDT- 2011/04/26 06:00
PHST- 2011/04/26 06:00 [entrez]
PHST- 2011/04/26 06:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - 10.1007/978-1-61779-080-5_26 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;731:321-32. doi: 10.1007/978-1-61779-080-5_26.