PMID- 21518038
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20191210
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 41
IP  - 6
DP  - 2011 Jun
TI  - Evaluation of two new recombinant guinea-pig lipocalins, Cav p 2 and Cav p 3, in 
      the diagnosis of guinea-pig allergy.
PG  - 899-908
LID - 10.1111/j.1365-2222.2011.03726.x [doi]
AB  - BACKGROUND: Guinea-pigs, classical laboratory animals now often kept as pets, 
      regularly elicit important allergic reactions. Two guinea-pig allergens have been 
      described previously to some extent; however, biomolecular and immunological data 
      are lacking. OBJECTIVE: To identify major guinea-pig allergens, produce them as 
      recombinant proteins and define their allergenic potential and clinical 
      importance in allergic patients. METHODS: Protein extracts were prepared from 
      various guinea-pig tissues and allergens were purified by ion exchange 
      chromatography. The N-termini of two immunoglobulin E (IgE)-reactive proteins 
      were determined and degenerate primers were designed for cDNA amplification by 
      RT-PCR. Allergenic properties of recombinant proteins were assayed by 
      immunoblotting, ELISA and mediator release assays. Specific IgE were quantified 
      in the sera of 26 guinea-pig-allergic patients. RESULTS: Major IgE-reactive 
      proteins were detected in submaxillary and harderian gland extracts. Two proteins 
      were identified and the cDNAs were cloned. The 17 kDa protein expressed in the 
      harderian gland corresponds to the previously described Cav p 2. The 19 kDa 
      protein, Cav p 3, is a new allergen expressed in the submaxillary gland. 
      Recombinant Cav p 2 and Cav p 3 were recognized by IgE antibodies from 65% and 
      54% of guinea-pig-allergic patients, respectively. Both proteins demonstrated 
      equivalent allergenic activity in the mediator release assays. CONCLUSION AND 
      CLINICAL RELEVANCE: Two major guinea-pig allergens, Cav p 2 and Cav p 3, have 
      been characterized and produced as recombinant proteins. Combined to guinea-pig 
      serum albumin, the new allergens proved to be valuable in the component-resolved 
      diagnosis of guinea-pig allergy.
CI  - (c) 2011 Blackwell Publishing Ltd.
FAU - Hilger, C
AU  - Hilger C
AD  - Laboratory of Immunogenetics and Allergology, CRP-Sante, Luxembourg, Luxembourg. 
      christiane.hilger@crp-sante.lu
FAU - Swiontek, K
AU  - Swiontek K
FAU - Kler, S
AU  - Kler S
FAU - Diederich, C
AU  - Diederich C
FAU - Lehners, C
AU  - Lehners C
FAU - Vogel, L
AU  - Vogel L
FAU - Vieths, S
AU  - Vieths S
FAU - Hentges, F
AU  - Hentges F
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110425
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Epitopes)
RN  - 0 (Lipocalins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Animals
MH  - Child
MH  - Child, Preschool
MH  - Cross Reactions/immunology
MH  - Epitopes/immunology
MH  - Female
MH  - Guinea Pigs/*immunology
MH  - Humans
MH  - Hypersensitivity/*diagnosis/immunology/metabolism
MH  - Immunoglobulin E/immunology/metabolism
MH  - Lacrimal Apparatus/immunology/metabolism
MH  - *Lipocalins/immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - *Recombinant Proteins/immunology
MH  - Salivary Glands/immunology/metabolism
MH  - Sequence Alignment
MH  - Young Adult
EDAT- 2011/04/27 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - 10.1111/j.1365-2222.2011.03726.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2011 Jun;41(6):899-908. doi: 10.1111/j.1365-2222.2011.03726.x. 
      Epub 2011 Apr 25.