PMID- 21518483
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20161125
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 19
IP  - 2
DP  - 2011 Apr
TI  - [Chimeric Ad5F35 adenoviral vector-mediated expression of mutant IkappaBalpha induces 
      apoptosis of leukemia cells].
PG  - 332-6
AB  - Constitutive activation of nuclear transcription factor-kappaB (NF-kappaB) exists in a 
      variety of leukemia, and induction of apoptosis through blocking NF-kappaB activation 
      may be an alternative strategy for leukemia treatment. The aim of this study was 
      to investigate the inducing effect of modified adenovirus 5-based adenovirus 
      vector (i.e. chimeric Ad5F35 Vec)-mediated expression of mutant IkappaBalpha (IkappaBalphaDN) on 
      apoptosis of HL-60 cells. The recombinant Ad5F35-IkappaBalphaDN Vec carrying IkappaBalphaDN cDNA 
      which deleted the first 1-70 amino acids coding sequences at 5' terminal of human 
      IkappaBalpha was transfected into HL-60 cells. The apoptosis, NF-kappaB DNA binding activity, 
      the expressions of IkappaBalpha, cIAP-2 and xIAP in HL-60 cells were detected by DNA 
      binding assay, flow cytometry, real-time quantitative polymerase chain reaction 
      and Western blot respectively. The results showed that apoptosis rates were 22.53 
      +/- 2.999%, 6.08 +/- 2.464% and 4.86 +/- 1.366% for Ad5F35-IkappaBalphaDN Vec-infected or blank 
      vector of Ad5F35-EGFP Vec-transfected and untransfected HL-60 cells respectively, 
      which showed a significant difference between Ad5F35-IkappaBalphaDN Vec-transfected and 
      untransfected cells (p < 0.001) and between Ad5F35-IkappaBalphaDN Vec-transfected and 
      Ad5F35-EGFP Vec-transfected cells (p < 0.001, p < 0.002), while NF-kappaB DNA binding 
      activity was decreased, the truncated IkappaBalpha was expressed, and IkappaBalpha mRNA 
      expression was up-regulated, but the expression of cIAP-2 and xIAP mRNA was 
      down-regulated after transduction for 48 hours. It is concluded that the chimeric 
      Ad5F35 Vec can effectively mediate the expression of IkappaBalphaDN cDNA in HL-60 cells, 
      leading to the inhibition of NF-kappaB DNA binding activity and inducing apoptosis of 
      HL-60 cells.
FAU - Wang, Guang-Ping
AU  - Wang GP
AD  - Department of Hematology, Central South University, Changsha, Hunan Province, 
      China. guangpingw@yahoo.com
FAU - Wang, Kai
AU  - Wang K
FAU - Xin, Hong-Ya
AU  - Xin HY
FAU - Duan, Zhao-Jun
AU  - Duan ZJ
FAU - Jing, Zhao-Zheng
AU  - Jing ZZ
FAU - Tan, San-Qing
AU  - Tan SQ
FAU - Qi, Zhen-Hua
AU  - Qi ZH
FAU - Chen, Fang-Ping
AU  - Chen FP
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
SB  - IM
MH  - Adenoviridae/genetics
MH  - *Apoptosis
MH  - Genetic Vectors
MH  - HL-60 Cells
MH  - Humans
MH  - I-kappa B Proteins/*genetics
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*genetics
MH  - Transfection
EDAT- 2011/04/27 06:00
MHDA- 2012/01/13 06:00
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
AID - 1009-2137(2011)02-0332-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Apr;19(2):332-6.