PMID- 21518509
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 19
IP  - 2
DP  - 2011 Apr
TI  - [Optimization of in vitro culture conditions for human amniotic epithelial cells 
      and expression of stem cell markers].
PG  - 464-8
AB  - This study was purposed to optimize the culture conditions of the human amniotic 
      epithelium cells (hAEC) in vitro, and detect the expression of hAEC pluripotent 
      markers. Amnion tissues were separated from the underlying chorion through the 
      spongy layer immediately after elective cesarean section of healthy pregnancy 
      women at term. After the subsequent exposure to trypsin digestion, hAEC were 
      cultured in DMEM with different supplements. The growth and proliferation 
      potential of hAEC was evaluated, and the expression of cultured hAEC pluripotent 
      markers was detected by using flow cytometry and immunohistochemistry methods. 
      The results indicated that when being cultured in the mediums similar to that of 
      embryonic stem cell culture supplemented with 10 ng/ml EGF, the hAEC grew better 
      and the time for passage was shortened. In addition, compared to other culture 
      conditions, under this condition, the cells could be passaged up to 5 times as 
      much without obvious morphological changes, and the pluripotent marker SSEA-4 was 
      detected in the cultured cells by flow cytometry. Meanwhile, the detection of 
      immunofluorescence showed the expression of vimentin in cultured hAEC was 
      strengthened as compared with primary cells. It is concluded that the culture 
      condition similar to that for embryonic stem cells supplemented with EGF 
      facilitates the proliferation and passage of hAEC in vitro.
FAU - Chen, You-Yi
AU  - Chen YY
AD  - Department of Obstetrics and Gynecology, The Fourth Military Medical University, 
      Xi'an, Shaanxi Province, China.
FAU - Lu, Yan
AU  - Lu Y
FAU - Wang, Ke
AU  - Wang K
FAU - Wang, Yan
AU  - Wang Y
FAU - Wu, Dong-Ying
AU  - Wu DY
FAU - Liu, Bin
AU  - Liu B
FAU - Yang, Ying
AU  - Yang Y
FAU - Lu, Shuang-Hong
AU  - Lu SH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Amnion/*cytology/metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Epithelial Cells/*cytology/metabolism
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Stem Cells/cytology/*metabolism
EDAT- 2011/04/27 06:00
MHDA- 2012/01/13 06:00
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
AID - 1009-2137(2011)02-0464-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Apr;19(2):464-8.