PMID- 21518788
OWN - NLM
STAT- MEDLINE
DCOM- 20110902
LR  - 20220409
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 79
IP  - 7
DP  - 2011 Jul
TI  - Monocyte chemoattractant protein 1 regulates pulmonary host defense via 
      neutrophil recruitment during Escherichia coli infection.
PG  - 2567-77
LID - 10.1128/IAI.00067-11 [doi]
AB  - Neutrophil accumulation is a critical event to clear bacteria. Since uncontrolled 
      neutrophil recruitment can cause severe lung damage, understanding neutrophil 
      trafficking mechanisms is important to attenuate neutrophil-mediated damage. 
      While monocyte chemoattractant protein 1 (MCP-1) is known to be a monocyte 
      chemoattractant, its role in pulmonary neutrophil-mediated host defense against 
      Gram-negative bacterial infection is not understood. We hypothesized that 
      MCP-1/chemokine (C-C motif) ligand 2 is important for neutrophil-mediated host 
      defense. Reduced bacterial clearance in the lungs was observed in MCP-1(-/-) mice 
      following Escherichia coli infection. Neutrophil influx, along with 
      cytokines/chemokines, leukotriene B(4) (LTB(4)), and vascular cell adhesion 
      molecule 1 levels in the lungs, was reduced in MCP-1(-/-) mice after infection. 
      E. coli-induced activation of NF-kappaB and mitogen-activated protein kinases in the 
      lung was also reduced in MCP-1(-/-) mice. Administration of intratracheal 
      recombinant MCP-1 (rMCP-1) to MCP-1(-/-) mice induced pulmonary neutrophil influx 
      and cytokine/chemokine responses in the presence or absence of E. coli infection. 
      Our in vitro migration experiment demonstrates MCP-1-mediated neutrophil 
      chemotaxis. Notably, chemokine receptor 2 is expressed on lung and blood 
      neutrophils, which are increased upon E. coli infection. Furthermore, our 
      findings show that neutrophil depletion impairs E. coli clearance and that 
      exogenous rMCP-1 after infection improves bacterial clearance in the lungs. 
      Overall, these new findings demonstrate that E. coli-induced MCP-1 causes 
      neutrophil recruitment directly via chemotaxis as well as indirectly via 
      modulation of keratinocyte cell-derived chemokine, macrophage inflammatory 
      protein 2, and LTB(4).
FAU - Balamayooran, Gayathriy
AU  - Balamayooran G
AD  - Laboratory of Lung Biology, Department of Pathobiological Sciences, and Center 
      for Experimental Infectious Disease Research, Louisiana State University Health 
      Sciences Center, New Orleans, Louisiana 70112, USA.
FAU - Batra, Sanjay
AU  - Batra S
FAU - Balamayooran, Theivanthiran
AU  - Balamayooran T
FAU - Cai, Shanshan
AU  - Cai S
FAU - Jeyaseelan, Samithamby
AU  - Jeyaseelan S
LA  - eng
GR  - R01 HL091958/HL/NHLBI NIH HHS/United States
GR  - R01 HL-091958/HL/NHLBI NIH HHS/United States
GR  - R01 HL-091958S1/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110425
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
EIN - Infect Immun. 2013 Nov;81(11):4323
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Bronchoalveolar Lavage Fluid/immunology/microbiology
MH  - Chemokine CCL2/*immunology/*metabolism
MH  - Chemokine CXCL2/analysis
MH  - Escherichia coli/*immunology
MH  - Escherichia coli Infections/*immunology/microbiology
MH  - Leukotriene B4/analysis
MH  - Lung/immunology/metabolism/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - *Neutrophil Infiltration
MH  - Pneumonia, Bacterial/*immunology/metabolism/microbiology
MH  - Vascular Cell Adhesion Molecule-1/analysis
PMC - PMC3191985
EDAT- 2011/04/27 06:00
MHDA- 2011/09/03 06:00
PMCR- 2012/01/01
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2011/09/03 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - IAI.00067-11 [pii]
AID - 0067-11 [pii]
AID - 10.1128/IAI.00067-11 [doi]
PST - ppublish
SO  - Infect Immun. 2011 Jul;79(7):2567-77. doi: 10.1128/IAI.00067-11. Epub 2011 Apr 
      25.